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Current Nutrition & Food Science

Author(s): Mohammad B. Zendeh, Vadood Razavilar*, Hamid Mirzaei and Khosrow Mohammadi

DOI: 10.2174/1573401315666181218151541

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Antimicrobial Effects of Zataria multiflora and Ocimum basilicum on Escherichia coli O157:H7 During Ripening of Traditional Lighvan Cheese

Page: [373 - 380] Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Background: Escherichia coli O157:H7 is one of the most common causes of contamination in Lighvan cheese processing. Using from natural antimicrobial essential oils is applied method to decrease the rate of microbial contamination of dairy products. The present investigation was done to study the antimicrobial effects of Z. multiflora and O. basilicum essential oils on survival of E. coli O157:H7 during ripening of traditional Lighvan cheese.

Methods: Leaves of the Z. multiflora and O. basilicum plants were subjected to the Clevenger apparatus. Concentrations of 0, 100 and 200 ppm of the Z. multiflora and 0, 50 and 100 ppm of O. basilicum essential oils and also 103 and 105 cfu/ml numbers of E. coli O157:H7 were used. The numbers of the E. coli O157:H7 bacteria were analyzed during the days 0, 30, 60 and 90 of the ripening period.

Results: Z. multiflora and O. basilicum essential oils had considerable antimicrobial effects against E. coli O157:H7. Using the essential oils caused decrease in the numbers of E. coli O157:H7 bacteria in 90th days of ripening (P <0.05). Using from Z. multiflora at concentration of 200 ppm can reduce the survival of E. coli O157:H7 in Lighvan cheese.

Conclusion: Using Z. multiflora and O. basilicum essential oils as good antimicrobial agents can reduce the risk of foodborne bacteria and especially E. coli O157:H7 in food products.

Keywords: Antimicrobial effects, Escherichia coli O157:H7, lighvan cheese, microbial contamination, Ocimum basilicum, Zataria multiflora.

Graphical Abstract

[1]
Aminifar M, Hamedi M, Emam-Djomeh Z, Mehdinia A. Investigation on proteolysis and formation of volatile compounds of Lighvan cheese during ripening. J Food Sci Technol 2014; 51(10): 2454-62.
[http://dx.doi.org/10.1007/s13197-012-0755-3] [PMID: 25328184]
[2]
Momtaz H, Dehkordi FS, Hosseini MJ, Sarshar M, Heidari M. Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran. Gut Pathog 2013; 5(1): 39.
[http://dx.doi.org/10.1186/1757-4749-5-39] [PMID: 24330673]
[3]
Momtaz H, Safarpoor Dehkordi F, Rahimi E, Ezadi H, Arab R. Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant’s meat. Meat Sci 2013; 95(2): 381-8.
[http://dx.doi.org/10.1016/j.meatsci.2013.04.051] [PMID: 23747633]
[4]
Momtaz H, Karimian A, Madani M, et al. Uropathogenic Escherichia coli in Iran: serogroup distributions, virulence factors and antimicrobial resistance properties. Ann Clin Microbiol Antimicrob 2013; 12(1): 8.
[http://dx.doi.org/10.1186/1476-0711-12-8] [PMID: 23627669]
[5]
Hemmatinezhad B, Khamesipour F, Mohammadi M, Safarpoor Dehkordi F, Mashak Z. Microbiological investigation of o-serogroups, virulence factors and antimicrobial resistance properties of Shiga toxin-producing Escherichia coli isolated from ostrich, turkey and quail neats. J Food Saf 2015; 35(4): 491-500.
[http://dx.doi.org/10.1111/jfs.12199]
[6]
Ranjbar R, Masoudimanesh M, Dehkordi FS, Jonaidi-Jafari N, Rahimi E. Shiga (Vero)-toxin producing Escherichia coli isolated from the hospital foods; virulence factors, o-serogroups and antimicrobial resistance properties. Antimicrob Resist Infect Control 2017; 6(1): 4.
[http://dx.doi.org/10.1186/s13756-016-0163-y] [PMID: 28074125]
[7]
Momtaz H, Safarpoor Dehkordi F, Taktaz T, Rezvani A, Yarali S. Shiga toxin-producing Escherichia coli isolated from bovine mastitic milk: serogroups, virulence factors, and antibiotic resistance properties. Sci World J 2012; 2012: 618709
[http://dx.doi.org/10.1100/2012/618709] [PMID: 23213293]
[8]
Momtaz H, Farzan R, Rahimi E, Safarpoor Dehkordi F, Souod N. Molecular characterization of Shiga toxin-producing Escherichia coli isolated from ruminant and donkey raw milk samples and traditional dairy products in Iran. Sci World J 2012; 2012: 231342
[http://dx.doi.org/10.1100/2012/231342] [PMID: 22919299]
[9]
Dehkordi FS, Yazdani F, Mozafari J, Valizadeh Y. Virulence factors, serogroups and antimicrobial resistance properties of Escherichia coli strains in fermented dairy products. BMC Res Notes 2014; 7(1): 217.
[http://dx.doi.org/10.1186/1756-0500-7-217] [PMID: 24708594]
[10]
Lekkas C, Kakouri A, Paleologos E, Voutsinas LP, Kontominas MG, Samelis J. Survival of Escherichia coli O157:H7 in Galotyri cheese stored at 4 and 12 degrees C. Food Microbiol 2006; 23(3): 268-76.
[http://dx.doi.org/10.1016/j.fm.2005.03.008] [PMID: 16943013]
[11]
Sajed H, Sahebkar A, Iranshahi M. Zataria multiflora Boiss. (Shirazi thyme)--an ancient condiment with modern pharmaceutical uses. J Ethnopharmacol 2013; 145(3): 686-98.
[http://dx.doi.org/10.1016/j.jep.2012.12.018] [PMID: 23266333]
[12]
Jain ML, Jain SR. Therapeutic utility of Ocimum basilicum var. album. Planta Med 1972; 22(1): 66-70.
[http://dx.doi.org/10.1055/s-0028-1099584] [PMID: 5083250]
[13]
Downes FP, Itō K. Compendium of methods for the microbiological examination of foods. 4th ed. Washington, DC: American Public Health Association 2001.
[http://dx.doi.org/10.2105/9780875531755]
[14]
Adams RP. Identification of essential oil components by gas chromatography/mass spectroscopy. CRC Press: USA 2007.
[15]
Vagionas K, Graikou K, Chinou I, Runyoro D, Ngassapa O. Chemical analysis and antimicrobial activity of essential oils from the aromatic plants Artemisia afra Jacq. and Leonotis ocymifolia (Burm. f.) Iwarsson var. raineriana (Vision1) Iwarsson growing in Tanzania. J Essent Oil Res 2007; 19(4): 396-400.
[http://dx.doi.org/10.1080/10412905.2007.9699314]
[16]
Vernozy-Rozand C, Ray-Gueniot S, Ragot C, et al. Prevalence of Escherichia coli O157:H7 in industrial minced beef. Lett Appl Microbiol 2002; 35(1): 7-11.
[http://dx.doi.org/10.1046/j.1472-765X.2002.01116.x] [PMID: 12081541]
[17]
Brichta-Harhay DM, Arthur TM, Bosilevac JM, Guerini MN, Kalchayanand N, Koohmaraie M. Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods. J Appl Microbiol 2007; 103(5): 1657-68.
[http://dx.doi.org/10.1111/j.1365-2672.2007.03405.x] [PMID: 17953577]
[18]
Le Breton M-H, Savoy-Perroud M-C, Diserens J-M. Validation and comparison of the Copan Milk Test and Delvotest SP-NT for the detection of antimicrobials in milk. Anal Chim Acta 2007; 586(1-2): 280-3.
[http://dx.doi.org/10.1016/j.aca.2006.11.060] [PMID: 17386724]
[19]
Meng F, Cargile BJ, Miller LM, Forbes AJ, Johnson JR, Kelleher NL. Informatics and multiplexing of intact protein identification in bacteria and the archaea. Nat Biotechnol 2001; 19(10): 952-7.
[http://dx.doi.org/10.1038/nbt1001-952] [PMID: 11581661]
[20]
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4): 564-82.
[http://dx.doi.org/10.1128/CMR.12.4.564] [PMID: 10515903]
[21]
Abedini A, Roumy V, Mahieux S, et al. Antimicrobial activity of selected Iranian medicinal plants against a broad spectrum of pathogenic and drug multiresistant micro-organisms. Lett Appl Microbiol 2014; 59(4): 412-21.
[http://dx.doi.org/10.1111/lam.12294] [PMID: 24888993]
[22]
Zomorodian K, Saharkhiz MJ, Rahimi MJ, et al. Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Pharmacogn Mag 2011; 7(25): 53-9.
[http://dx.doi.org/10.4103/0973-1296.75902] [PMID: 21472080]
[23]
Joshi RK. Chemical composition and antimicrobial activity of the essential oil of Ocimum basilicum L. (sweet basil) from Western Ghats of North West Karnataka, India. Anc Sci Life 2014; 33(3): 151-6.
[http://dx.doi.org/10.4103/0257-7941.144618] [PMID: 25538349]
[24]
Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 2005; 26(5): 343-56.
[http://dx.doi.org/10.1016/j.ijantimicag.2005.09.002] [PMID: 16323269]
[25]
Ehsani A, Hashemi M, Naghibi SS, Mohammadi S, Khalili Sadaghiani S. Properties of Bunium persicum essential oil and its application in Iranian white cheese against Listeria monocytogenes and Escherichia coli O157: H7. J Food Saf 2016; 36(4): 563-70.
[http://dx.doi.org/10.1111/jfs.12277]
[26]
Sadeghi E, Mohammadi A, Jamilpanah M, Bashiri M, Bohlouli S. Antimicrobial effects of Mentha pulegium essential oil on Listeria monocytogenes in Iranian white cheese. J food Quality Hazards Control 2016; 3(1): 20-4.
[27]
Shahbazi Y. Ziziphora clinopodioides essential oil and nisin as potential antimicrobial agents against Escherichia coli O157: H7 in doogh (Iranian yoghurt drink). J Pathogens 2015; 2015: 176024
[http://dx.doi.org/10.1155/2015/176024] [PMID: 26783466]
[28]
Al-Reza SM, Rahman A, Kang SC. Chemical composition and inhibitory effect of essential oil and organic extracts of Cestrum nocturnum L. on food-borne pathogens. Int J Food Sci Technol 2009; 44(6): 1176-82.
[http://dx.doi.org/10.1111/j.1365-2621.2009.01939.x]
[29]
Dehkordi FS, Parsaei P, Saberian S, et al. Prevalence study of Theileria annulata by comparison of four diagnostic techniques in southwest Iran. Bulg J Vet Med 2012; 15(2): 123-30.
[30]
Madahi H, Rostami F, Rahimi E, Safarpoor Dehkordi F. Prevalence of enterotoxigenic Staphylococcus aureus isolated from chicken nugget in Iran. Jundishapur J Microbiol 2014; 7(8): e10237
[PMID: 25485044]
[31]
Safarpoor Dehkordi F, Barati S, Momtaz H, Hosseini Ahari SN, Nejat Dehkordi S. Comparison of shedding, and antibiotic resistance properties of Listeria monocytogenes isolated from milk, feces, urine, and vaginal secretion of bovine, ovine, caprine, buffalo, and camel species in Iran. Jundishapur J Microbiol 2013; 6(3): 284-94.
[32]
Momtaz H, Davood Rahimian M, Safarpoor Dehkordi F. Identification and characterization of Yersinia enterocolitica isolated from raw chicken meat based on molecular and biological techniques. J Appl Poult Res 2013; 22(1): 137-45.
[http://dx.doi.org/10.3382/japr.2012-00549]
[33]
Ghorbani F, Gheisari E, Dehkordi FS. Genotyping of vacA alleles of Helicobacter pylori strains recovered from some Iranian food items. Trop J Pharm Res 2016; 15(8): 1631-6.
[http://dx.doi.org/10.4314/tjpr.v15i8.5]
[34]
Rahimi E, Sepehri S, Safarpoor Dehkordi F, Shaygan S, Momtaz H. Prevalence of yersinia species in traditional and commercial dairy products in isfahan province, iran. Jundishapur J Microbiol 2014; 7(4): e9249
[http://dx.doi.org/10.5812/jjm.9249] [PMID: 25147698]
[35]
Dehkordi FS, Khamesipour F, Momeni M. Brucella abortus and Brucella melitensis in Iranian bovine and buffalo semen samples: the first clinical trial on seasonal, Senile and geographical distribution using culture, conventional and real-time polymerase chain reaction assays. Kafkas Univ Vet Fak Derg 2014; 20(6): 821-8.
[36]
Dehkordi FS, Haghighi N, Momtaz H, Rafsanjani MS, Momeni M. Conventional vs real-time PCR for detection of bovine herpes virus type 1 in aborted bovine, buffalo and camel foetuses. Bulg J Vet Med 2013; 16(2): 102-11.
[37]
Rahimi E, Yazdanpour S, Dehkordi FS. Detection of Toxoplasma gondii antibodies in various poultry meat samples using enzyme linked immuno sorbent assay and its confirmation by polymerase chain reaction. J Pure Appl Microbiol 2014; 8(1): 421-7.
[38]
Dehkordi FS, Valizadeh Y, Birgani TA, Dehkordi KG. Prevalence Study of Brucella melitensis and Brucella abortus in cow’s milk using dot enzyme linked immuno sorbent assay and duplex polymerase chain reaction. J Pure Appl Microbiol 2014; 8(2): 1065-9.