Abstract

Introduction: Lactate Minimum Test (LMT) identifies a sustainable exercise intensity, in which an equilibrium is observed between production and clearance of blood lactate and the hormone influence during this physiological moment.

Objective: The present study aimed to identify the levels of LM and hormones after caffeine consumption and exercise Stress Test (ST) in diabetic rats.

Methods: This study was composed of 24 animals, of 60 days, allocated into four groups: Control, Diabetic, Caffeine, and Diabetes+Caffeine. The Diabetes model was induced by intraperitoneal administration of 120 mg/kg of alloxan. On the test day, 6 mg/kg of caffeine were administrated 30 minutes before the exercise Stress Test (ST) protocol. During the ST animals underwent a Stress Test (ST), in which they performed forced swimming (until exhaustion) tie to loads of 13% Body’s Weight (BW). The incremental phase of LM began with an initial load of 4% Body’s Weight (BW) and increased 0.5% every 5 min. Lactate concentration was measured 5, 7 and 9 min (mmol/L) after ST. The Incremental Progressive Test (IPT) involved swimming with loads of 4.0, 4.5, 5.0, 5.5, 6.0, and 7.0% of BW, for 5min with each. Blood samples were collected by a caudal puncture to subsequent lactate and hormone assay.

Results: Performance time and lactate concentration of hyperlactatemia test, as well as Lactate Minimum (LM) and Lactate (LAC) concentration after the progressive test presented a significant difference when comparing the levels of the control group with caffeine and diabetic group (p<0.05).

Conclusion: It is suggested that caffeine improves lactate clearance and hormonal steady state condition of diabetic animals after hyperlactacidemia and physical exercise maintenance.

Keywords: Caffeine, conditioning, hyperlactatemia, lactate, metabolism, stress test.