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Current Nutrition & Food Science

Author(s): Meena Verma, Abhishake Saxena, Punesh Sangwan*, Imran Sheikh, Vinod Kumar and Harcharan S. Dhaliwal

DOI: 10.2174/1573401316999200901172600

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Phytase Mediated Beneficial Impact on Nutritional Quality of Biofortified Wheat Genotypes

Page: [490 - 500] Pages: 11

  • * (Excluding Mailing and Handling)

Abstract

Background: Biofortification has been proposed as an intervention towards alleviation of micronutrient deficiency in the population of developing countries. However, the presence of anti- nutritional factor phytic acid in staple cereals chelates divalent cations and decreases their bioavailability for monogastric animals. Thus, the use of phytase enzyme for hydrolysing phytate-P and enhancing the amount of free divalent cations is of great importance.

Methods: In this study, two phytases i.e. APF1 phytase from fungal source and commercial wheat phytase were supplemented with flours of biofortified wheat genotypes and their impact on food quality parameters was accessed. Since commercial wheat phytase is costly, it was used as known phytase to compare the application of APF1 phytase. The phytic acid content was reduced in the range of 70 to 84% with APF1 phytase and 79 to 89% with the wheat phytase as compared to untreated samples, respectively. In contrast to phytate, the dialyzability of important micronutrients Fe and Zn enhanced in the range of 21.9 to 48% and 39.5 to 96% with APF1 phytase and, 6.10 to 30% and 23.2 to 81% with wheat phytase, over untreated samples, respectively.

Results and Discussion: A decrease in tannin content was observed in the range of 8 to 23% and 7 to 23% after treatment with APF1 and wheat phytase, respectively. The phytase treatment has resulted in increased soluble protein content and inorganic phosphate content to different level over untreated samples.

Conclusion: The study revealed that APF1 phytase was comparatively more effective for enhanced nutritional quality of wheat flour through phytase supplementation for its food based applications.

Keywords: Micronutrient, dialyzability, biofortification, phytase, phytic acid, inorganic phosphate.

Graphical Abstract

[1]
Reddy N, Pierson MD, Sathe SK, Salunkhe D. Phytates in cereals and legumes. CRC Press 1989.
[2]
Loewus F. Biosynthesis of phytate in food grains and seeds. In: Food phytates. USA: CRC Press 2002; pp. 53-61.
[3]
Raboy V. Seeds for a better future: ‘low phytate’ grains help to overcome malnutrition and reduce pollution. Trends Plant Sci 2001; 6(10): 458-62.
[http://dx.doi.org/10.1016/S1360-1385(01)02104-5] [PMID: 11590064]
[4]
Chitra U, Singh U, Rao PV. Phytic acid, in vitro protein digestibility, dietary fiber, and minerals of pulses as influenced by processing methods. Plant Foods Hum Nutr 1996; 49(4): 307-16.
[http://dx.doi.org/10.1007/BF01091980] [PMID: 8983057]
[5]
Chen Q. Determination of phytic acid and inositol pentakisphosphates in foods by high-performance ion chromatography. J Agric Food Chem 2004; 52(15): 4604-13.
[http://dx.doi.org/10.1021/jf035294x] [PMID: 15264889]
[6]
Al-Wahsh IA, Horner HT, Palmer RG, Reddy MB, Massey LK. Oxalate and phytate of soy foods. J Agric Food Chem 2005; 53(14): 5670-4.
[http://dx.doi.org/10.1021/jf0506378] [PMID: 15998131]
[7]
Lestienne I, Icard-Vernière C, Mouquet C, Picq C, Trèche S. Effects of soaking whole cereal and legume seeds on iron, zinc and phytate contents. Food Chem 2005; 89: 421-5.
[http://dx.doi.org/10.1016/j.foodchem.2004.03.040]
[8]
Das A, Raychaudhuri U, Chakraborty R. Cereal based functional food of Indian subcontinent: a review. J Food Sci Technol 2012; 49(6): 665-72.
[http://dx.doi.org/10.1007/s13197-011-0474-1] [PMID: 24293685]
[9]
Butler LG. Antinutritional effects of condensed and hydrolyzable tannins. Plant polyphenols. USA: Springer 1992; pp. 693-8.
[http://dx.doi.org/10.1007/978-1-4615-3476-1_40]
[10]
Chung K-T, Wong TY, Wei C-I, Huang Y-W, Lin Y. Tannins and human health: a review. Crit Rev Food Sci Nutr 1998; 38(6): 421-64.
[http://dx.doi.org/10.1080/10408699891274273] [PMID: 9759559]
[11]
Davidsson L, Galan P, Kastenmayer P, et al. Iron bioavailability studied in infants: the influence of phytic acid and ascorbic acid in infant formulas based on soy isolate. Pediatr Res 1994; 36(6): 816-22.
[http://dx.doi.org/10.1203/00006450-199412000-00024] [PMID: 7898991]
[12]
Kumari A, Chugh LK, Kumar V, Nagar S, Kharor N. Sheenu. Phytase from pearl millet: its partial purification and Characterization. Forage Res 2020; 46(1): 78-83.
[13]
Kumar V, Sangwan P, Verma AK, Agrawal S. Molecular and biochemical characteristics of recombinant β-propeller phytase from Bacillus licheniformis strain PB-13 with potential application in aquafeed. Appl Biochem Biotechnol 2014; 173(2): 646-59.
[http://dx.doi.org/10.1007/s12010-014-0871-9] [PMID: 24687556]
[14]
Kumar V, Singh G, Sangwan P, Verma AK, Agrawal S. Cloning, sequencing, and in silico analysis of β-propeller phytase bacillus licheniformis strain PB-13. Biotechnol Res Int 2014; 2014: 841353.
[http://dx.doi.org/10.1155/2014/841353] [PMID: 24864215]
[15]
Greiner R, Konietzny U. Phytase for food application. Food Technol Biotechnol 2006; 44(2): 125-40.
[16]
Kumar V, Sinha AK, Makkar HP, Becker K. Dietary roles of phytate and phytase in human nutrition: a review. Food Chem 2010; 120: 945-59.
[http://dx.doi.org/10.1016/j.foodchem.2009.11.052]
[17]
Fujita J, Yamane Y, Fukuda H, et al. Production and properties of phytase and acid phosphatase from a sake koji mold, Aspergillus oryzae. J Biosci Bioeng 2003; 95(4): 348-53.
[http://dx.doi.org/10.1016/S1389-1723(03)80066-X] [PMID: 16233418]
[18]
Rezaei M, Borbor S, Zaghari M, Teimouri A. Effect of phytase supplementation on nutrients availability and performance of broiler chicks. Int J Poult Sci 2007; 6(1): 55-8.
[http://dx.doi.org/10.3923/ijps.2007.55.58]
[19]
Zaghari M. Evaluation of using phytase nutrient equivalency values for layer hens and broiler chickens. J Agric Sci 2009; 11: 57-66.
[20]
Saxena A, Verma M, Singh B, et al. Characteristics of an acidic phytase from Aspergillus aculeatus APF1 for dephytinization of biofortified wheat genotypes. Appl Biochem Biotechnol 2020; 191: 679-94.
[http://dx.doi.org/10.1007/s12010-019-03205-9]
[21]
Gao Y, Shang C, Maroof M, et al. A modified colorimetric method for phytic acid analysis in soybean. Crop Sci 2007; 47: 1797-803.
[http://dx.doi.org/10.2135/cropsci2007.03.0122]
[22]
Kasim AB, Edwards HM Jr. The analysis for inositol phosphate forms in feed ingredients. J Sci Food Agric 1998; 76: 1-9.
[http://dx.doi.org/10.1002/(SICI)1097-0010(199801)76:1<1::AID-JSFA922>3.0.CO;2-9]
[23]
Miller DD, Schricker BR, Rasmussen RR, Van Campen D. An in vitro method for estimation of iron availability from meals. Am J Clin Nutr 1981; 34(10): 2248-56.
[http://dx.doi.org/10.1093/ajcn/34.10.2248] [PMID: 6794346]
[24]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54.
[http://dx.doi.org/10.1016/0003-2697(76)90527-3] [PMID: 942051]
[25]
Saxena V, Mishra G, Saxena A, Vishwakarma K. A comparative study on quantitative estimation of tannins in Terminalia chebula, Terminalia belerica, Terminalia arjuna and Saraca indica using spectrophotometer. Asian J pharm Clin Res 2013; 6: 148-9.
[26]
Kalsi HK, Singh R, Dhaliwal HS, Kumar V. Phytases from Enterobacter and Serratia species with desirable characteristics for food and feed applications. 3 Biotech 2016; 6: 64.
[27]
Kaur R, Saxena A, Sangwan P, Yadav AN, Kumar V, Dhaliwal HS. Production and characterization of a neutral phytase of Penicillium oxalicum EUFR-3 isolated from Himalayan region. Nusantara Bioscie 2017; 9: 68-76.
[http://dx.doi.org/10.13057/nusbiosci/n090112]
[28]
Dost K, Tokul O. Determination of phytic acid in wheat and wheat products by reverse phase high performance liquid chromatography. Anal Chim Acta 2006; 558: 22-7.
[http://dx.doi.org/10.1016/j.aca.2005.11.035]
[29]
Gupta RK, Gangoliya SS, Singh NK. Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains. J Food Sci Technol 2015; 52(2): 676-84.
[http://dx.doi.org/10.1007/s13197-013-0978-y] [PMID: 25694676]
[30]
Vashishth A, Ram S, Beniwal V. Cereal phytases and their importance in improvement of micronutrients bioavailability. 3 Biotech 2017; 7: 42.
[31]
Knorr D, Watkins TR, Carlson BL. Enzymatic reduction of phytate in whole wheat breads. J Food Sci 1981; 46: 1866-9.
[http://dx.doi.org/10.1111/j.1365-2621.1981.tb04506.x]
[32]
Nielsen AV, Tetens I, Meyer AS. Potential of phytase-mediated iron release from cereal-based foods: a quantitative view. Nutrients 2013; 5(8): 3074-98.
[http://dx.doi.org/10.3390/nu5083074] [PMID: 23917170]
[33]
Greiner R, Konietzny U. Improving enzymatic reduction of myo‐inositol phosphates with inhibitory effects on mineral absorption in black beans (Phaseolus vulgaris var. preto). J Food process Pres 1999; 23: 249-61.
[34]
Konietzny U, Greiner R, Jany K-D. Biochemistry and application of phytases from spelt and rye. Getreide Mehl und Brot Germany 1995; 49(5): 265-271.
[35]
Kumar V, Yadav AN, Verma P, et al. β-Propeller phytases: diversity, catalytic attributes, current developments and potential biotechnological applications. Int J Biol Macromol 2017; 98: 595-609.
[http://dx.doi.org/10.1016/j.ijbiomac.2017.01.134] [PMID: 28174082]
[36]
Khan N, Zaman R, Elahi M. Effect of processing on the phytic acid content of wheat products. J Agric Food Chem 1986; 34: 1010-2.
[http://dx.doi.org/10.1021/jf00072a019]
[37]
Tangkongchitr U. Phytic Acid. Ii: Its Fate During Breadmaking 1981.
[38]
Dahiya S, Singh N. Isolation and biochemical characterization of a novel phytase producing bacteria Bacillus cereus isolate MTCC 10072. Int J Microbial Resource Technol 2014; 2: 1-5.
[39]
Hurrell RF, Reddy MB, Juillerat M-A, Cook JD. Degradation of phytic acid in cereal porridges improves iron absorption by human subjects. Am J Clin Nutr 2003; 77(5): 1213-9.
[http://dx.doi.org/10.1093/ajcn/77.5.1213] [PMID: 12716674]
[40]
Lung S-C, Chan W-L, Yip W, Wang L, Yeung EC, Lim BL. Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization. Plant Sci 2005; 169: 341-9.
[http://dx.doi.org/10.1016/j.plantsci.2005.03.006]
[41]
Düngelhoef M, Rodehutscord M, Spiekers H, Pfeffer E. Effects of supplemental microbial phytase on availability of phosphorus contained in maize, wheat and triticale to pigs. Anim Feed Sci Technol 1994; 49: 1-10.
[http://dx.doi.org/10.1016/0377-8401(94)90076-0]
[42]
Bali A, Satyanarayana T. Microbial phytases in nutrition and combating phosphorus pollution. Everyman’s Sci 2001; 4: 207-9.
[43]
Wolters M, Diepenmaat H, Hermus R, Voragen A. Relation between in vitro availability of minerals and food composition: a mathematical model. J Food Sci 1993; 58: 1349-55.
[http://dx.doi.org/10.1111/j.1365-2621.1993.tb06181.x]
[44]
Argyri K, Theophanidi E, Kapna A, et al. Iron or zinc dialyzability obtained from a modified in vitro digestion procedure compare well with iron or zinc absorption from meals. Food Chem 2011; 127(2): 716-21.
[http://dx.doi.org/10.1016/j.foodchem.2011.01.005] [PMID: 23140725]
[45]
Bikker P, Jongbloed AW, Thissen JT. Meta-analysis of effects of microbial phytase on digestibility and bioavailability of copper and zinc in growing pigs. J Anim Sci 2012; 90(Suppl. 4): 134-6.
[http://dx.doi.org/10.2527/jas.53798] [PMID: 23365307]
[46]
Zaghari M, Avazkhanllo M, Ganjkhanlou M. Reevaluation of male broiler zinc requirement by dose-response trial using practical diet with added exogenous phytase. J Agric Sci Technol 2015; 17(2): 333-43.
[47]
Adeola O, Lawrence BV, Sutton AL, Cline TR. Phytase-induced changes in mineral utilization in zinc-supplemented diets for pigs. J Anim Sci 1995; 73(11): 3384-91.
[http://dx.doi.org/10.2527/1995.73113384x] [PMID: 8586598]
[48]
Srivastava R. Antinutrients restraining biofortification. In: Biofortification Food Crops. New Delhi: Springer 2016; pp. 333-48.
[http://dx.doi.org/10.1007/978-81-322-2716-8_24]
[49]
Mroz Z, Jongbloed AW, Kemme PA. Apparent digestibility and retention of nutrients bound to phytate complexes as influenced by microbial phytase and feeding regimen in pigs. J Anim Sci 1994; 72(1): 126-32.
[http://dx.doi.org/10.2527/1994.721126x] [PMID: 8138479]
[50]
Vielma J, Ruohonen K, Gabaudan J, Vogel K. Top-spraying soybean meal-based diets with phytase improves protein and mineral digestibilities but not lysine utilization in rainbow trout, Oncorhynchus mykiss (Walbaum). Aquacult Res 2004; 35: 955-64.
[http://dx.doi.org/10.1111/j.1365-2109.2004.01106.x]
[51]
Cheng ZJ, Hardy RW. Effects of extrusion and expelling processing, and microbial phytase supplementation on apparent digestibility coefficients of nutrients in full-fat soybeans for rainbow trout (Oncorhynchus mykiss). Aquac 2003; 218: 501-14.
[http://dx.doi.org/10.1016/S0044-8486(02)00458-1]
[52]
Lestienne I, Besançon P, Caporiccio B, Lullien-Péllerin V, Tréche S. Iron and zinc in vitro availability in pearl millet flours (Pennisetum glaucum) with varying phytate, tannin, and fiber contents. J Agric Food Chem 2005; 53(8): 3240-7.
[http://dx.doi.org/10.1021/jf0480593] [PMID: 15826084]
[53]
Alonso R, Aguirre A, Marzo F. Effects of extrusion and traditional processing methods on antinutrients and in vitro digestibility of protein and starch in faba and kidney beans. Food Chem 2000; 68: 159-65.
[http://dx.doi.org/10.1016/S0308-8146(99)00169-7]
[54]
Matuschek E, Towo E, Svanberg U. Oxidation of polyphenols in phytate-reduced high-tannin cereals: effect on different phenolic groups and on in vitro accessible iron. J Agric Food Chem 2001; 49(11): 5630-8.
[http://dx.doi.org/10.1021/jf0108157] [PMID: 11714370]
[55]
Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev 1998; 56(11): 317-33.
[http://dx.doi.org/10.1111/j.1753-4887.1998.tb01670.x] [PMID: 9838798]
[56]
Papadopoulou A, Frazier RA. Characterization of protein–polyphenol interactions Trends Food Sci Tech 2004; 15: 186-90.
[http://dx.doi.org/10.1016/j.tifs.2003.09.017]
[57]
Bedford MR. Exogenous enzymes in monogastric nutrition—their current value and future benefits. Anim Feed Sci Technol 2000; 86: 1-13.
[http://dx.doi.org/10.1016/S0377-8401(00)00155-3]
[58]
Barrier-Guillot B, Casado P, Maupetit P, Jondreville C, Gatel F, Larbier M. Wheat phosphorus availability: 2—in vivo study in broilers and pigs; relationship with endogenous phytasic activity and phytic phosphorus content in wheat. J Sci Food Agric 1996; 70: 69-74.
[http://dx.doi.org/10.1002/(SICI)1097-0010(199601)70:1<69::AID-JSFA466>3.0.CO;2-C]
[59]
Qian Y, Roland G, Xu C. Why is China different from Eastern Europe? Perspectives from organization theory. Eur Econ Rev 1999; 43: 1085-94.
[http://dx.doi.org/10.1016/S0014-2921(98)00116-0]