Quality Assurance of Immunohistochemistry for Breast Cancers using a Whole-slide Imaging System, Specified Software and Cell Lines

Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Cite As

Abstract

An auto-stainer (AS), whole-slide imaging system (WSI), and computer software were used as quality assurance tools for breast cancer immunohistochemistry (IHC).

Cell lines, such as MCF-7 for estrogen receptors and progesterone receptors, and SKBR- 3 for Topo2a, were co-stained with samples as IHC control.

Combinatory usage of AS, WSI, computer software and cell lines can reduce deviation in each IHC step and is beneficial for IHC quality assurance.