Abstract
SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.
Abstract
Modeling of the main metabolic pathways such as glycolysis, TCA cycle, pentose phosphate (PP) pathway, and anaplerotic pathways is considered, where the fluxes obtained by computer simulation can be used to compute the specific ATP production rate, the specific CO2 production rate, and the specific NAD(P)H production rate. The specific ATP production rate thus computed can be used for the estimation of the specific growth rate. The model can be further extended for catabolite regulation by incorporating the effects of transcription factors such as Cra and cAMP-Crp, where acetate overflow metabolism and co-consumption of multiple sugars can be clarified by this modeling approach. Modeling for anaerobic fermentation is also considered, where aerobic/ anaerobic switch can be made by incorporation of the roles of ArcA/B and Fnr. Modeling for NH3 assimilation pathways is then considered, where this may be combined with the main metabolism to simulate nitrogen regulation at various C/N ratios. Finally, amino acid synthetic pathways are considered for lysine production.
Keywords:
Modeling, kinetics, computer simulation, dynamic simulation, gene knockout mutant, Escherichia coli, catabolite regulation, co-consumption of multiple carbon sources, nitrogen metabolism, anaerobic fermentation, transcription factors, lysine fermentation.
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Authors:Bentham Science Books