Methods to Determine Enzymatic Activity

Author(s): Bernardo Dias Ribeiro and Maria Alice Zarur Coelho

DOI: 10.2174/9781608053001113010006

Assays of Peroxidase Activity

Pp: 27-38 (12)

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Abstract

Peroxidases are enzymes that use various peroxides as electron acceptors in oxidation, and are widespread in nature. Their activities are usually determined by spectrophotometric methods, which a condensation or oxidation product is formed in the presence of H2O2, or by titrimetric methods, using potassium permanganate or sodium thiosulfate, which measure free hydrogen peroxide in solution.

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