Antibodies Applications and New Developments

Author(s): Abby J. Jackson, Matthew R. Sobansky and David S. Hage

DOI: 10.2174/978160805264611201010156

Download Download PDF Flyer Cite As
Principles and Applications of Immunoaffinity Chromatography

Pp: 156-174 (19)

Buy Chapters
Add to Cart

* (Excluding Mailing and Handling)

  • * (Excluding Mailing and Handling)

Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

Immunoaffinity Chromatography (IAC) is a type of liquid chromatography that uses the highly specific binding of antibodies for the retention of a target. This approach can be used in a myriad of techniques for the selective purification, concentration and/or analysis of target compounds. This chapter discusses the basic components of an IAC method, including the support, stationary phase, immobilization technique, and proper sample application and elution conditions. Several applications of IAC are also considered. These applications include immunoextraction, immunodepletion, chromatographic immunoassays, and post-column immunodetection. A wide range of targets that have been isolated or measured by these techniques are listed. Related methods based on antibody mimics are also discussed.

Keywords:

Antibodies, Immunoaffinity Chromatography

Recommended Chapters

We recommend

Favorable 70-S: Investigation Branching Arrow

Authors:Bentham Science Books

Cite as