Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach

Author(s): Ishfaq Ahmad Rather* and Rashid Ali * .

DOI: 10.2174/9789815165913124010004

Colorimetric Indicator Displacement Assays (CIDAs)

Pp: 35-59 (25)

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* (Excluding Mailing and Handling)

  • * (Excluding Mailing and Handling)

Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

Owing to the unique advantages of naked eye color detection in the sensing of various important analytes, colorimetric indicator displacement assay (C-IDA) has received its recognition as the principal evolutionary extension of IDA. This chapter, besides dealing with all conceptual details of C-IDA, also offers basic criteria for the development and classification of chromogenic sensing ensembles. A plethora of examples in order to elucidate the C-IDA sensing mechanism for the detection of anionic, cationic, and neutral analytes via prominent UV-vis spectral changes have been revealed. Besides, the recognition of several biologically essential analytes viz. phosphate and saccharides have also been discussed in detail through numerous examples. The authors believe that this chapter on C-IDA will offer new dimensions to the design and fabrication of IDA-based colorimetric sensors.

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