Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach

Author(s): Ishfaq Ahmad Rather* and Rashid Ali * .

DOI: 10.2174/9789815165913124010006

Metal Complexing Indicator Displacement Assays (M-IDAs)

Pp: 91-118 (28)

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* (Excluding Mailing and Handling)

  • * (Excluding Mailing and Handling)

Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

As a sub-class of colorimetric indicator displacement assays (C-IDAs) as well as fluorimetric indicator displacement assays (F-IDAs), metal indicator displacement assays (M-IDAs) involve primarily the coordination of metal ions with a target receptor and subsequent coordination of colorimetric or fluorimetric indicator with complexed metal ions and also with the target receptor. The chronological addition of an interested analyte brings the displacement of the colorimetric or fluorimetric indicator from the metal ion and the receptor, which in turn illustrates the binding affair either through colorimetric or fluorescence Turn-ON/OFF response. The authors have illustrated the role of M-IDAs in the detection of various indispensable analytes through a vast number of examples and deduced their need in various domains from future perspectives. The authors are of the opinion that this chapter will bring new dimensions to metal coordination chemistry in general and sensor development in particular.

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