Micropropagation of Medicinal Plants

Author(s): S. Anitha * .

DOI: 10.2174/9789815196146124010004

In vitro Propagation Protocol of Tylophora indica (Burm.f.)Merrill

Pp: 37-68 (32)

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  • * (Excluding Mailing and Handling)

Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

Tylophora indica (Burm.f.) Merrill is one of the most commonly used medicinal plants with bioactive alkaloid-rich secondary metabolites. This plant is used to treat asthma, dysentery, whooping cough, rheumatic pains, jaundice, and cancer. Rapid exploitation of this plant in natural habitats and poor regeneration methods, which are not in pace with those of destruction, make tissue culture methods a viable option to be used as a method of conservation. In the present chapter, tissue culture protocols have been reported till now as the best viable means in the rapid multiplication of T. indica. Sterilization protocols, callus induction and somatic embryogenesis methods, and direct and indirect organogenesis used by different researchers in mass propagation and acclimatization are given in detail. The present chapter gives an insight into the hormones needed and the response of the explants, which will be helpful for those who want to propagate this medicinal plant under in vitro conditions.

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