Microsurgery In Liver Research

Author(s): Maria-Angeles Aller, Maria-Paz Nava, Marta Mendez and Jaime Arias

DOI: 10.2174/978160805068010901010071

Extraction Techniques of Blood, Bile and Lymph Samples

Pp: 71-93 (23)

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Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

The extraction of multiple body fluids samples is frequently necessary when research studies involving laboratory rodents are developed. Thus, in the current chapter different techniques for collecting blood, bile and lymph samples are described. Methods for obtaining blood without anesthesia of the rat, as well as methods requiring anesthesia are explained. In addition, blood withdrawal by using long-term catheterization of blood vessels is described. Techniques of bile sample collection include catheterization of the common bile duct. Finally, techniques of lymph sample collection through catheterization of the thoracic lymph duct and mesenteric lymph duct are explained.

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