Abstract

Due to the chemical complexity and wide concentration range of lipids in biological samples, it is necessary to apply different analytical strategies to identify and quantify lipid species and amounts. In this book chapter, we mainly introduced the techniques, workflow, and applications of lipidomics in food nutrition and health. First, we mainly introduced the common lipidomics techniques, such as direct infusion mass spectrometry-based techniques, chromatographic separation mass spectrometry-based techniques, mass spectrometry imaging, and nuclear magnetic resonance. Second, we described the common lipidomics workflow, including sample preparation, MS data acquisition, and data processing. Third, we mainly discussed the application of lipidomics in food nutrition and health. Finally, we briefly summarized and discussed the future perspectives of lipidomics. All these discussions suggested that lipidomics could ensure food quality, examine dietary lipid nutrition, and prevent and detect diseases.