Frontiers in Natural Product Chemistry

Author(s): Ibtisam Abdul Wahab, Noel F. Thomas, Jean-Frederic F. Weber, Khalijah Awang, A. Hamid A. Hadi and Pascal Richomme

DOI: 10.2174/978160805212710501010177

Efficient HPLC Procedures for Natural Product Isolation: Application to Phenolics from Timber

Pp: 177-184 (8)

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Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

We undertook the (re-)investigations of the chemical composition of the phenolic contents of Malaysian timber species. In order to efficiently isolate these constituents, we developed semi-automated separation procedures. It includes the extensive use of HPLC systems, operated at low or high pressure, in conjunction with automatic injection and automatic fraction collection. The efficiency of this procedure is demonstrated by the isolation of an array of oligostilbenoids from the heartwood of chengal (Neobalanocarpus heimii, Dipterocarpaceae), when only a single compound was isolated by the previous investigators.

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