Recent Advances in Biotechnology

Author(s): Manar M. Hozyen* and Islam M. Saadeldin * .

DOI: 10.2174/9789815051667122050009

Advances in the Human Preimplantation Embryonic Culture System

Pp: 153-173 (21)

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* (Excluding Mailing and Handling)

  • * (Excluding Mailing and Handling)

Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

The embryonic culture system is the main part of in-vitro embryonic development. The culture system consists of chemical factors such as the chemical composition of the culture media and physical factors such as temperature, pH, oil overlay and, incubation systems. The optimal culture conditions are needed to support embryonic development in-vitro, increase pregnancy, implantation rates, and decrease the level of pregnancy loss. Any suboptimality in culture conditions may impair embryonic development and affect subsequent viability. In this chapter, we will focus on the current culture system in the field of assisted reproductive technology (ART), passing by current culture media formulations, deficiencies, culture devices used, and the areas that need further investigations. 

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