Surface Enhanced Raman Spectroscopy: Biosensing and Diagnostic Technique for Healthcare Applications

Author(s): Jagjiwan Mittal* and Robin Kumar

DOI: 10.2174/9789815039115121010004

State of the Art: Raman Vibrational Spectroscopy and Surface Enhanced Raman Spectroscopy

Pp: 1-20 (20)

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* (Excluding Mailing and Handling)

  • * (Excluding Mailing and Handling)

Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

Raman spectroscopy depends on inelastic scattering of photons, known as
Raman scattering. It uses monochromatic light using a laser and determines vibrational
modes of molecules. This technique is commonly used for the identification of
molecules by providing its structural fingerprint. Due to very low inelastic scattering,
however, signals obtained by Raman spectroscopy are inherently weak and the problem
is more with visible light. These weak Raman signals can be used by amplifying them
by the method known as surface enhanced Raman spectroscopy (SERS). SERS is a
powerful vibrational spectroscopy technique that allows for highly sensitive structural
detection of low concentration analytes. The current chapter summarizes the basics of
Raman spectroscopy and SERS, instrumentation, mechanisms differences and
applications.

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