Frontiers in Enzyme Inhibition

Author(s): Elsa Cherian and G Baskar

DOI: 10.2174/9789811460821120010007

Enzyme Biosensors Based on Enzyme Inhibition for Pesticide and Heavy Metal Detection

Pp: 59-72 (14)

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Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

A biosensor is an analytical apparatus, which connects a biologically sensitive and selective component and a physiochemical transducer. Biologically sensitive elements include organisms, tissues, cells, etc. and the transducer includes electrochemical, optical, thermal or mechanical signals which are received and converted into a measurable signal. There are different categories of biosensors based on the principle of working. Some of them are electrochemical, amperometric, thermometric, optical, microbial and immunosensors. Biosensors are broadly used in different areas like the food industry, fermentation industry, pharmaceutical industry, etc. The present chapter explains the use of different types of biosensors which are based upon enzyme inhibition, as an investigative and diagnostic tool. Some of the enzyme inhibitors such as pollutants are strongly associated with human as well as environmental health, so these have to be monitored with strong significance. Thus enzyme inhibition based enzyme biosensors will be a precious tool for very fast performing and accurate for the above applications.

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