Abstract
SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.
Abstract
Genomic DNA extraction is the starting point for various downstream molecular biology applications viz. PCR, restriction analysis, hybridisation etc. Numerous problems like DNA degradation, co-isolation of viscous polysaccharides, polyphenols and other secondary metabolites causing damage to DNA, inhibiting restriction enzymes, DNA polymerases etc, are routinely encountered during DNA isolation from plants. Quinone compounds resulting from oxidation of polyphenols lead brown the DNA preparations and can also damage proteins and DNA`s due to their oxidizing properties. This results in a poor yield of high molecular weight DNA. The protocol below explains the extraction of DNA via the CTAB method, involving three major steps viz lysis of cell wall and membranes, extraction of genomic DNA and precipitation of DNA.
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Authors:Bentham Science Books