Fluorescence Microscopy in Life Sciences

Author(s): Juan Carlos Stockert and Alfonso Blazquez-Castro

DOI: 10.2174/9781681085180117010014

Lipids

Pp: 336-358 (23)

Buy Chapters

* (Excluding Mailing and Handling)

  • * (Excluding Mailing and Handling)

Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

In this chapter, the uses of most known fluorescent compounds for the microscopic visualization of lipids are described. Particular emphasis is given to the nature of substrates, fixing requirements, dye solubility, and examples of lipophilic stains. Probes for visualizing membranous or lipidic structures in live cells will be also described in Chapter 15.5. Hydrophobic azo and anthraquinone dyes are applied industrially to color fats, paints, plastic resins, rubbers, varnishes and waxes. Some of these dyes are employed to detect lipid droplets and fat-rich components of tissues. Several fat-soluble dyes, particularly bisazo Sudan dyes and Nile red have been routinely used in visualizing lipids [1-3]. Relevant natural and synthetic polyenes such as fluorescent antifungal antibiotics and unsaturated fatty acids will be also described.

Recommended Chapters

We recommend

Favorable 70-S: Investigation Branching Arrow

Authors:Bentham Science Books