Fluorescence Microscopy in Life Sciences

Author(s): Juan Carlos Stockert and Alfonso Blazquez-Castro

DOI: 10.2174/9781681085180117010013

Luminescent Transition Metal Probes

Pp: 283-335 (53)

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Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

In this chapter, the fundamentals in regard to the use of transition metal complexes (TMCs) as luminescent probes will be presented. These metal complexes (MCs) between organic ligands and transition metal cations present many interesting properties that make them very suitable for a number of biomedical applications. First, a brief introduction to TMCs and their photophysics and photochemistry will be provided. Then, some particular applications of selected transition metals (TMs) in the field of fluorescence microscopy will be presented on a case-per-case basis. The relevant role played by lanthanide elements and their complexes in fluorescence microscopy, especially time-resolved, will be introduced. Some uses of actinide MCs will be presented as well.

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