Abstract
SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.
Abstract
Spectral changes of dyes related to pH and metal coordination have now increasing importance as analytical tools. Changes in the light absorption or emission that are characteristics of some dyes and reagents at different pH values or in the presence of metal ions, as well as their microscopical uses are described in this Chapter. Regarding histochemical and histophysiological detection, important metal cations are Mg, Ca, Fe, Zn, etc., with methodological procedures being spot tests, spectral studies and microscopic observations.
Keywords:
Alizarin, Aluminum salts, Anthraquinones, Calcein, Calcification markers, Carmine, Chelating reagents, Crown ethers, Flavonols, Fluorescent pH probes, Intracellular pH, Polychromatic fluorescence, Pyranine, Ratio imaging.
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Authors:Bentham Science Books