Fluorescence Microscopy in Life Sciences

Author(s): Juan Carlos Stockert and Alfonso Blazquez-Castro

DOI: 10.2174/9781681085180117010006

Physical Fundamentals of Luminescence

Pp: 22-60 (39)

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Abstract

SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.

Abstract

The fundamental physical and chemical processes responsible for luminescence will be introduced and explained in this chapter. The classical division of luminescence into Fluorescence and Phosphorescence will be presented along the similarities and differences between these two processes. Starting with a simple introduction to light and electromagnetic waves and moving to atomic and molecular systems, the text will allow the reader to intuitively understand why physical systems absorb and emit light. This chapter represents the theoretical foundations on which the rest of the book is built upon, the platform from which all other chapters and processes explained therein can be adequately understood and apprehended.

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