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SHS investigation development is considered from the geographical and historical viewpoint. 3 stages are described. Within Stage 1 the work was carried out in the Department of the Institute of Chemical Physics in Chernogolovka where the scientific discovery had been made. At Stage 2 the interest to SHS arose in different cities and towns of the former USSR. Within Stage 3 SHS entered the international scene. Now SHS processes and products are being studied in more than 50 countries.
Abstract
High-Performance Size-Exclusion Chromatography (HPSEC) is widely used for the determination of the molar mass and size distribution of biopolymers in aqueous or organic solvents. Elements of the theory of fractionation by HPSEC, column calibration and online light scattering detection are given, showing that coupling HPSEC with multi-angle laser light scattering detection makes it easier to obtain molar mass distributions since light (MALLS) scattering gives the weight-average molar mass at each elution volume of the chromatogram. Some applications of HPSEC-MALLS for the macromolecular characterization of starches, glycogens, dextrans, celluloses, hemicelluloses, β-glucans, pectins, gums, alginates, carrageenans, chitosans, lignins, proteins and peptides are also presented.
Keywords:
Cellulose, Chitosan, Gum, High-Performance Size-Exclusion Chromatography, Lignin, Multi-angle laser light scattering, Polysaccharide, Protein, Starch, Static and dynamic light scattering.
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Authors:Bentham Science Books