Abstract

Background: This study evaluated the biological properties of twelve 1,3-thiazolidin-4- ones by assessing antifungal and antibacterial activities.

Methods: All compounds were tested against three fungi and two bacteria. The lowest concentration that produced a ≥50% inhibition of the growth of yeast in comparison with positive control was identified as MIC₅₀. Compound 1b showed the best antifungal result against Candida albicans with MIC₅₀ 100 μg/ml (52.38% of growth inhibition). In the antifungal Rhodotorula assay, two compounds showed good antifungal activity with MIC₅₀ 100 μg/ml (1f: 82.91% and 1i: 61.91%). For C. parapsilosis compound 1i has MIC₅₀ 200 μg/ml.

Results and Conclusion: No antibacterial activity was found for Staphylococcus aureus and Escherichia coli at the highest concentration tested (400 μg/ml). The cytotoxicity study for three compounds was carried out against primary astrocyte cells and no toxic effect was found at 400 μM. This study discovered at least two potential compounds that will guide further chemical modification to search for better results.

Keywords: 4-thiazolidinones, 1, 3-benzodioxol, candida albicans, candida parapsilosis, rhodotorula mucilaginosa, astrocytes.

Graphical Abstract