Author(s): Craig Giragossian, Jennifer LaPerle, Rachel E. Kosa and Sarah Gillian
Page: [45 - 53] Pages: 9
* (Excluding Mailing and Handling)
Enzyme kinetic parameters for midazolam were time-dependent in human liver microsomes, under initial velocity conditions. Vmax and Km decreased up to 3.7 and 3.1-fold, respectively, for 10 min compared to 1 min incubations. Mathematical models describing the relationship between inactivation and the time-dependency of enzyme kinetic parameter estimates were derived and discussed.
Keywords: Enzyme kinetics, midazolam, mechanism based inactivation, time-dependent inactivation
