Author(s): Jiankun Qie, Jinbo Ma, Liangyou Wang, Xiaoyu Xu, Jianquan Zheng, Sijian Dong, Jianwei Xie, Huixian Sun, Wenxia Zhou, Chunhui Qi, Xiunan Zhao, Yongxiang Zhang and Keliang Liu
Page: [232 - 340] Pages: 109
* (Excluding Mailing and Handling)
Site-specific mono-PEGylations were performed in different conformational regions of Thymosin alpha 1 (Tα1) by introducing one cysteine residue into the chosen site and coupling with thiol-specific mPEG-MAL reagent. Results demonstrated that PEGylated sites and regions influenced the conformations and pharmacokinetic profiles of the peptide greatly with following order: α-helix, β-turn, random coil and terminals, but little on the immunoactivity.
Keywords: Site-specific PEGylation, thymosin alpha 1, immunoactivity
