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Letters in Drug Design & Discovery

Author(s): Kurt W. Vogel, Bryan D. Marks, Kevin R. Kupcho, Kevin L. Vedvik and Tina M. Hallis

DOI: 10.2174/157018008785777261

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Facile Conversion of FP to TR-FRET Assays using Terbium Chelates:Nuclear Receptor Competitive Binding Assays as Examples

Page: [416 - 422] Pages: 7

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Abstract

Time-resolved FRET (TR-FRET) assays offer advantages over fluorescence polarization (FP) assays including reduced optical interference from compound autofluorescence or precipitation, and a greater ability to resolve potencies of tight-binding ligands. Here, we demonstrate the facile conversion of FP assays to a TR-FRET format using nuclear receptor ligand binding assays as examples.

Keywords: Fluorescence polarization, Time-resolved fret, Terbium chelates, Nuclear receptors