Abstract

To increase the peptide diversity of phage display libraries, we examined the incorporation of amino acid analogues into bacteriophage using auxotrophic E. coli strains. Fluorinated tryptophan analogues and norleucine were efficiently incorporated into phage particles. Phage display of unnatural amino acids may have wide applications in the drug discovery process.

Keywords: combinatorial chemistry, phage display, peptide, unnatural amino acid