Author(s): Donald Perillat, Hong Tan, Robert Zuk, Scott Lockard, Janette Phi-Wilson, Michael Howes, Juan Gamez, Tim Ellis, Huddee Ho, Kevin Du, Michael Recknor, Greg Carricato, Joy Concepcion, Lian-She Zhao, Ram Varma, Weilei Ma, Bettina Heidecker, Pu Li, Jing Wei, Henrik Persson, Danfeng Yao, Sae Choo, Charles Wartchow and Krista Witte
Page: [791 - 800] Pages: 10
* (Excluding Mailing and Handling)
The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.
Download PDF Flyer