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Combinatorial Chemistry & High Throughput Screening

Author(s): Khayam ul Haq, Shazia Noreen, Sheikh A. Sehgal, Rana A. Tahir, Amjad Essa, Awais Ihsan, Mahreen ul Hassan, Waheed Iqbal, Masroor Hussain, Hamid Hussain and Sami Siraj*

DOI: 10.2174/1386207323666200402083234

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Association of Virulence Genes with Antibiotic Resistance in Pakistani Uropathogenic E. coli Isolates

Page: [517 - 524] Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Background: Escherichia coli various strains can cause alarmingly serious infections. Countries like Pakistan harbour the class of bacteria with one of the highest rates of resistance, but very little has been done to explore their genetic pool.

Objectives: This study was designed to find out the frequency of virulence genes of Uropathogenic E. coli and their association with antibiotic resistance along with the evolutionary adaptation of the selected gene through the phylogenetic tree.

Methods: Isolates from 120 urinary tract infected patients were collected. Antibiotic sensitivity was detected by the disk diffusion method and DNA extraction was done by the boiling lysis method followed by PCR-based detection of virulence genes. The final results were analysed using the chi-square test.

Results: The isolates were found to be least susceptible to nalidixic acid, followed by ampicillin, cotrimoxazole, cefotaxime, ciprofloxacin, aztreonam, amoxicillin, gentamycin, nitrofurantoin and imipenem. The iucC was the most common virulence gene among the resistant isolates. About 86% of the collected samples were found to be multi-drug resistant. Statistical analysis revealed a significant association between the iucC gene and resistance to ampicillin (P=0.03) and amoxicillin (P=0.04), and also between fimH and resistance to aztreonam (P=0.03).

Conclusion: This study unravels the uncharted virulence genes of UPEC in our community for the very first time. We report a high frequency of the iucC and fimH virulence genes. This, along with their positive association with resistance to beta-lactam antibiotics in the studied community, indicates their important role in the development of complicated UTIs.

Keywords: Urinary tract infection, uropathogenic E. coli, virulence, antibiotic resistance, iucC gene, beta-lactam antibiotics.

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