Abstract
Background: H+/K+ ATPase a protein present in the gastric parietal cells is a better target
for the prevention and treatment of gastric ulcer. Plant flavonoids have been reported to elicit
anti-ulcer activity by inhibiting the proton pump as well as by antioxidant defense mechanism.
Methods: Chloroform fraction of hydro-alcoholic extract of passion fruit was screened for proton
pump inhibitory assay using goat parietal cell. In-silico computational docking studies were carried
out using Glide program in order to validate the inhibitory action of selected constituents.
Results: The flavonoid rich fruit possess a promising radical scavenging activity against DPPH.
10.41μg/mL is sufficient to inhibit 50% of ATPase enzyme activity. A synergistic activity was also
achieved by the fruit with sub-effective doses of lansoprazole. Fenton's oxidation induced by H2O2
was also blunted by the fruit extract.
Conclusion: The in-vitro and in-silico findings indicated that, passion fruit can be a good dietary
supplement for the prevention and management of ulcer.
Keywords:
Passion fruit, ulcer, H+/K+ ATPase, flavonoids, oxidation, glide program.
Graphical Abstract
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