<i>Protocols used in Molecular Biology</i> is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

<p> This reference provides information about recent trends in bryology in parts of India, tropical rainforests and arctic regions. Bryophytes are the earliest land plants and quite fascinating in their overall diversity. All through its history, bryological study has contributed considerably to the field of plant sciences, for instance, the discovery of sex chromosomes in plants. The study of bryophytes is fundamental to our understanding of land plant evolution, and the latest progress in molecular phylogenetics and genomics have given researchers a clear depiction of land colonization of plants and subsequent terrestrial progression. Ecologically, the importance of bryophytes for the participation in biogeochemical cycles, in particular carbon cycle is now appreciated. Further, there has been an escalating interest in the conservation biology of bryophytes. The contributors have put forward holistic information regarding current research scenario of bryology in a range of environments to readers learning about research in applied bryology. The compilation of reviews presents reported findings related to various aspects of the subject, such as, conservation, diversity, tissue culture, bio-monitoring, computational bryology, molecular bryology, and species. Botanists and bryologists will receive updated information that will be valuable for their research work. The reader-friendly text is also suitable for beginners in applied plant science. </p><p> Recent Advances in Botanical Science provides updated research and reviews on topics related to plant biology, genetics, taxonomy and ecology. The series is a useful resource for readers interested in applied plant science. </p>

This monograph focuses on the actions exerted by sex hormones, 17β-estradiol and testosterone, in skeletal muscle tissue. An important consideration of this volume is the fact that both estrogen receptors (ERs) and androgen receptors (ARs) are ubiquitously expressed and, as a result, steroid hormones affect growth and different cell functions in several organs. Moreover, ERs and ARs may have a non-classical pattern of intracellular localizations, raising complexity to the functional roles of estradiol and testosterone. </p><p> Readers will find key information about the role of sex hormones in mitochondrial physiology and their relation with ageing, apoptosis, and sarcopenia. Chapters integrate important points with the latest information on the subject, including work of leading researchers studying the cellular and molecular mechanisms underlying the age-linked changes in muscle tissue while highlighting the role of satellite cells. </p><p> Furthermore, the book presents a chapter about phytoestrogens (compounds which are structurally very similar to estrogen 17β-estradiol) and their selective action on sex steroid receptors (specifically, they have a higher affinity for ERβ receptors than ERα receptors). </p><p> The book is recommended reading for scientists and clinicians involved in the field of medical and health sciences as well as for scholarly readers (students of biochemistry and medicine) who are interested in the molecular mechanism of cellular apoptosis regulated by steroid hormones.

This eBook presents a summary of central aspects of sialobiology (i.e., the study of sialic acid and its relevance to biology). The importance of substitution by the sugar sialic acid and the role played by sialylated structures (eg. glycoproteins, glycolipids, glycoconjugates) in immune recognition, neural cell growth, embryogenesis and disease development including microbial pathogenesis and cancer progression, has become well-established. Since 1995, the field of sialobiology has expanded greatly as many of the key enzymes involved in sialic acid biosynthesis, as well as the vast majority of sialic acid binding lectins involved in immune recognition, have only been cloned, characterised and structural eluciated after the publication of earlier works on the subject. This e-book also covers these recent developments. Chapters in this e-book have been contributed by eminent sialobiologists. Therefore, a book of this nature is timely and will prove to be a definitive volume with a high impact in this field for glycobiologists and cell biologists.

Polarity is a physico-chemical property that characterizes the electromagnetic stability of a protein and can be used to predict its plausible pathogenic action. For this reason, polarity is regarded as a major factor in most mathematical-computational algorithms that seek to characterize peptides and proteins. The Polarity Index Method makes it possible to reproduce the main classification of peptide proteins found in different databases, with a high degree of discriminative efficiency. Polarity Index In Proteins is a brief monograph that explains the foundations of the polarity index method and presents examples of the application of this method for identifying the structural and functional relationships of different types of proteins (including cell penetrating peptides and natively unfolded proteins). The monograph is divided into sections that cover basic protein biochemistry, the computational mathematical foundations of the polarity index method, the application of the method on different protein structures, and the evaluation of the results of famous experiments on biogenesis (Miller &amp;amp; Urey, Fox &amp;amp; Harada, Rode) by the same method. Polarity Index In Proteins serves as an essential handbook for students and researchers in the field of bioinformatics, proteomics as well as for studies on the role of proteins in the origin of life.

This e-book provides readers a short introductory MATLAB® course oriented towards various collaborative areas of biotechnology and bioscience. The text concentrates on MATLAB® fundamentals and gives examples of its application for various problems in computational biology, molecular biology, biokinetics, biomedicine, bioinformatics, and biotechnology. MATLAB® is presented with examples and applications to various school-level and advanced life science / bioengineering problems - from growing populations of microorganisms and population dynamics, reaction kinetics and reagent concentrations, predator-prey models, to data fitting and time series analysis. The book is divided into 6 chapters containing material carefully selected and tailored to teaching several groups of biotechnology students. The topics are presented in a manner that allows readers to proceed sequentially on the strength of the preceding material. Primary MATLAB® for Life Sciences: A Guide for Beginners is essentially a concise and comprehensive text that provides an easy grasp and to-the-point access to the MATLAB® tool to the community of life sciences and bioengneering undergraduates and specialists.

Multiple biotic and abiotic environmental factors may constitute stresses that affect plant growth and yield in crop species. Advances in plant physiology, genetics, and molecular biology have greatly improved our understanding of plant responses to stresses. This book details on technologies that have emerged during the past decade and have been useful in studying the multigenicity of the plant abiotic stress response. Upstream molecular mechanisms are involved in the plant response to abiotic stress, above all in the regulation of timings and amount of specific stress responses. Post-transcriptional mechanisms based on alternative splicing and RNA processing, as well as RNA silencing define the actual transcriptome supporting the stress response. Beyond protein phosphorylation, other post-translational modifications like ubiquitination and sumoylation regulate the activation of pre-existing molecules to ensure a prompt response to stress factors. The text in this book deals with the importance of -omics approaches like Genomics, Metabolomics and Proteomics in abiotic stress tolerance. Large scale analytical approaches provide detailed information about the structure and complexity of signaling networks, identify subsets of genes or activities that are correlated to given stress factors and reveal unexpected or previously uncharacterized biochemical interactions. To the best of our knowledge no book on -Omics studies in relation to Plant Abiotic Stress Tolerance is available in the market. This book should therefore be a valuable asset for the readers.

Events of reproduction occurring from meiotic resumption of the immature oocyte up to its exit from the second meiotic block following activation will be revealed. Morphological modifications of the oocyte during maturation will be related to signal transduction mechanisms involving primary or secondary messengers. A detailed view will be addressed to genetic and epigenetic control of oocyte maturation. At fertilization, reciprocal gamete activation and novel knowledge related to sperm factor and cascade mechanisms occurring in the oocyte will be focused. A detailed description of ion currents occurring during fertilization will depict another point of view of oocyte activation mechanisms, further supporting for their complexity. Finally, all basic information related to this short time lapse will be considered in relation to clinical application of assisted reproductive technologies. New frontiers, such as stem cells and cloning technologies, will be analyzed and future applications and improvements will be hypothesised.

Brazil has emerged as a significant financial and industrial power in recent times. Brazil is poised to become a significant player in the field of biotechnology, internationally, by taking advantage of circumstances not available in other countries, particularly its native biodiversity. This will, in turn, have an effect on commercial and entrepreneurial opportunities in the region. Topics covered in this text include adjustments that must be made in the regulatory framework to assure the success of business investment. This investment is crucial for training R&D scientists and developing new technologies. The book also covers a debate on transgenic plants which had political ramifications in the region and slowed the adoption rate of genetically modified organisms by almost a decade. The opportunities for commercialization of recombinant DNA technologies in the country are also presented. Opportunities and Limitations For Biotechnology Innovation In Brazil presents a concise overview of the biotechnology industry in Brazil and will be of great interest to a wide range of readers including researchers, biotechnology graduates, as well as both local and international investors.