Generic placeholder image

Current Pharmaceutical Analysis

Author(s): Xuliang Huang, Youjia Wu , Xiaoying Zhang , Bing Chen, Hongbin Luo, Xinhua Lin , Peiying Shi * and Hong Yao*

DOI: 10.2174/1573412915666190329220647

DownloadDownload PDF Flyer Cite As
Simultaneous Determination of Five Irdoid Glycosides and Three Flavonoid Glycosides in Hedyotis Diffusa Wild by UPLC-UV with Ultrasound-Assisted Extraction

Page: [808 - 818] Pages: 11

  • * (Excluding Mailing and Handling)

Abstract

Background: Hedyotis diffusa is one of the most extensively used Chinese herbs for the treatment of varieties of cancers in the folk of China. The ingredients of the preparation are still not very clear and the quality control method of the herb medicine requires further improvement.

Introduction: In this paper, the quantitative methods for the quality control of H. diffusa were originally developed by using UPLC-UV.

Methods: Using the five irdoid glycosides and three flavonoid glycosides previously obtained from H. diffusa as index components, referring to 10-Acetyl Scandoside Methyl Ester (ASME), Scandoside (SD), Deacetyl Aspemloside (DA), Asperulosidic Acid (AA), Asperuloside (AD), Qurecetin-3-OSophoroside (QS), Quercetin-3-O-[2-(6-O-E-Sinapoyl-β-D-Glucopyranosyl]-β-D-Glucopyranosyl (QESGG), and Quercetin-3-O-[2-O-(6-O-E-Feruloyl)-β-D-Glucopyranosyl]-β-Glucopyanoside (QEFGG), the extraction conditions, including solvents, durations, and cycles were optimized firstly. Subsequently, a new UPLC-UV method was originally established to simultaneously determine the eight ingredients in the Chinese herbal medicine.

Results: The validated method exhibited good linearity (R2 > 0.999), repeatability (RSD < 3.01%), intra- and inter-day precision (RSD <6.19%) and recoveries (96.31% to 104.22%) for the simultaneous determination of the eight ingredients studied and had been used for the quality analysis of 6 batches of H. diffusa sameples.

Conclusion: The established UPLC-UV method could serve as a rapid and effective tool for quality evaluation of the herbal medicine, H. diffusa.

Keywords: Hedyotis diffusa, quantitative determination, UPLC-UV, irdoid glycoside, flavonoid glycoside, extraction.

Graphical Abstract

[1]
Sun, G.; Wei, L.; Feng, J.; Lin, J.; Peng, J. Inhibitory effects of Hedyotis diffusa Willd on colorectal cancer stem cells. Oncol. Lett., 2016, 11(6), 3875-3881.
[2]
Li, Y.; Zhang, J.; Min, D.; Hongyan, Z.; Lin, N.; Li, Q.S. Anticancer effects of 1,3-Dihydroxy-2-Methylanthraquinone and the ethyl acetate fraction of Hedyotis diffusa Willd against HepG2 carcinoma cells mediated via apoptosis. PLoS One, 2016, 11(4)e151502
[3]
Zhang, P.; Zhang, B.; Gu, J.; Hao, L.; Hu, F.; Han, C. The study of the effect of Hedyotis diffusa on the proliferation and the apoptosis of the cervical tumor in nude mouse model. Cell Biochem. Biophys., 2015, 72(3), 783-789.
[4]
Yoshida, Y.; Wang, M.Q. LIU, J.N.; Shan, B.E.; Yamashita, U. Immunomodulating activity of Chinese medicinal herbs and Oldenlandia diffusa in particular. Int. J. Immunopharmacol., 1997, 19(7), 359-370.
[5]
Kuo, Y.J.; Lin, J.P.; Hsiao, Y.T.; Chou, G.L.; Tsai, Y.H.; Chiang, S.Y.; Lin, J.G.; Chung, J.G. Ethanol extract of Hedyotis diffusa Willd affects immune responses in normal Balb/c mice in vivo. In Vivo, 2015, 29(4), 453.
[6]
Kim, Y.; Park, E.J.; Kim, Y.; Kim, S.R.; Kim, Y.Y. Neuroprotective constituents from Hedyotis diffusa. J. Nat. Prod., 2001, 64(1), 75-78.
[7]
Ganbold, M.; Barker, J.; Ren, M.; Jones, L.; Carew, M. Cytotoxicity and bioavailability studies on a decoction of Oldenlandia diffusa and its fractions separated by HPLC. J. Ethnopharmacol., 2010, 131(2), 396-403.
[8]
Ling, Y.Z. Separation and content determination of polysassharides in Hedyotis diffusa Willd. Biotechnology , 2005, 15, 48-50.
[9]
Cheung, H.Y.; Cheung, S.H.; Law, M.L.; Lai, W.P. Simultaneous determination of key bioactive components in Hedyotis diffusa by capillary electrophoresis. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2006, 834, 195-198.
[10]
Liu, K.; Yan, L.Q.; Yao, G.C.; Guo, X. Estimation of p-coumaric acid as metabolite of E-6-O-p-coumaroyl scandoside methyl ester in rat plasma by HPLC and its application to a pharmacokinetic study. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2006, 831, 303-306.
[11]
Liang, Z.T.; Jiang, Z.H.; Leung, K.S.Y.; Zhao, Z.Z. Determination of iridoid glucosides for quality assessment of Herba oldenlandiae by high-performance liquid chromatography. Chem. Pharm. Bull. (Tokyo), 2006, 54, 1131-1137.
[12]
Li, P.F.; Li, J.J.; Liu, Y.; Guo, Y.; Liu, Y.; Ren, Yi. Quantitative and qualitative determination of gallic acid in Hedyotis diffusa Willd. extract and its anti-lung cancer NCI-H460 cell activity. Biomed. Res.-India, 2017, 28(12), 5280-5284.
[13]
Li, D.; Schmitz, O.J. Comprehensive two-dimensional liquid chromatography tandem Diode Array Detector (DAD) and accurate mass QTOF-MS for the analysis of flavonoids and iridoid glycosides in Hedyotis diffusa. Anal. Bioanal. Chem., 2015, 407(1), 231-240.
[14]
Liu, E.H.; Zhou, T.; Li, G.B.; Li, J.; Huang, X.N.; Pan, F.; Gao, N. Characterization and identification of iridoid glucosides, flavonoids and anthraquinones in Hedyotis diffusa by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry. J. Sep. Sci., 2015, 35(2), 263-272.
[15]
Nguyen, D.T.; Guillarme, D.; Heinisch, S.; Barrioulet, M.P.; Rocca, J.L.; Rudaz, S.; Veuthey, J.L. High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature. J. Chromatogr. A, 2007, 1167(1), 76-84.
[16]
Swartz, M.E. UPLC: An introduction and review. J. Liq. Chromatogr. Relat. Technol., 2005, 28(7-8), 1253-1263.
[17]
Plumb, R.; Castro-Perez, J.; Granger, J.; Beattie, I.; Joncour, K.; Wright, A. Ultra-performance liquid chromatography coupled to quadrupole-orthogonal time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom., 2010, 18(19), 2331-2337.
[18]
Zhang, Y.; Chen, F.F.; Sang, J. Green approach for sample preparation and determination of anthocyanins from Lycium ruthenicum Murr. using a beta-cyclodextrin-based extraction method coupled with UPLC-DAD Analysis. Food Anal. Methods, 2018, 11(8), 2141-2148.
[19]
Lamichhane, R.; Kim, S.G.; Poudel, A.; Sharma, D.; Lee, K.H.; Poudel, P.; Pandeya, P.R. Jung. H.-J. Show less Identification of flavonoids from Cheilanthes albomarginata Clarke and their simultaneous determination and quantification by UPLC/DAD Method. J. Liq. Chromatogr. Relat. Technol., 2015, 38(19), 1713-1721.