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Current Pharmaceutical Analysis

Author(s): Qinghua Weng, Yichuan Chen, Zuoquan Zhong, Qianqian Wang, Lianguo Chen and Xianqin Wang*

DOI: 10.2174/1573412915666190329215736

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Determination of Shanzhiside Methylester in Rat Plasma by Uplc-Ms/Ms and its Application to a Pharmacokinetic Study

Page: [960 - 966] Pages: 7

  • * (Excluding Mailing and Handling)

Abstract

Introduction: In this study, we used UPLC-MS/MS to detect shanzhiside methylester in rat plasma, and investigated its pharmacokinetics in rats.

Materials and Methods: Diazepam was utilized as an internal standard (IS), and acetonitrile precipitation method was used to process the plasma samples. Chromatographic separation was achieved using a UPLC BEH C18 column using mobile phase of methanol-0.1 % formic acid with gradient elution. Electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive ionization was applied.

Results: The results indicated that within the range of 5-4000 ng/mL, linearity of shanzhiside methylester in rat plasma was acceptable (r>0.995), and the lower limit of quantification (LLOQ) was 5 ng/mL. Intra-day and inter-day precision RSD of shanzhiside methylester in rat plasma were lower than 14%. Accuracy range was between 87.3 % and 109.1 %, and matrix effect was between 99.2% and 106.3%.

Conclusion: The method was successfully applied in the pharmacokinetics of shanzhiside methylester in rats after intravenous administration.

Keywords: Shanzhiside methylester, pharmacokinetics, rat, UPLC-MS/MS, plasma, Diazepam.

Graphical Abstract

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