Prunella vulgaris: A Comprehensive Review of Chemical Constituents, Pharmacological Effects and Clinical Applications

Page: [359 - 369] Pages: 11

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Abstract

Prunella vulgaris (PV) is a perennial herb belonging to the Labiate family and is widely distributed in the northeastern Asian countries such as Korea, Japan, and China. It is reported to display diverse biological activities including anti-microbial, anti-cancer, and anti-inflammation as determined by in vitro or in vivo studies. So far, about 200 compounds have been isolated from PV plant and a majority of these have been characterized mainly as triterpenoids, sterols and flavonoids, followed by coumarins, phenylpropanoids, polysaccharides and volatile oils. This review summarizes and analyzes the current knowledge on the chemical constituents, pharmacological activities, mechanisms of action and clinical applications of the PV plant including its potential as a future medicinal plant. Although some of the chemical constituents of the PV plant and their mechanisms of action have been investigated, the biological activities of many of these remain unknown and further clinical trials are required to further enhance its reputation as a medicinal plant.

Keywords: Prunella vulgaris, constituent, activity, application, review, triterpenoids.

[1]
Chen ST, Dou J, Temple R, Agarwal R, Wu KM, Walker S. New therapies from old medicines. Nat Biotechnol 2008; 26(10): 1077-83.
[2]
Kakeya H. Natural products-prompted chemical biology: phenotypic screening and a new platform for target identification. Nat Prod Rep 2016; 33(5): 648-54.
[3]
Hu YX, Yu CH, Wu F, et al. Antihepatofibrotic effects of aqueous extract of Prunella vulgaris on carbon tetrachloride-induced hepatic fibrosis in rats. Planta Med 2016; 82(1-2): 97-105.
[4]
Huang R, Zhao M, Yang X, et al. Effects of Prunella vulgaris on the mice immune function. PLoS One 2013; 8(10)e77355
[5]
Ru M, Wang K, Bai Z, et al. A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L. Sci Rep 2017; 7(1): 4892.
[6]
Collins NH, Lessey EC, DuSell CD, et al. Characterization of antiestrogenic activity of the Chinese herb, Prunella vulgaris, using in vitro and in vivo (Mouse Xenograft) models. Biol Reprod 2009; 80(2): 375-83.
[7]
Choi JH, Han EH, Hwang YP, et al. Suppression of PMA-induced tumor cell invasion and metastasis by aqueous extract isolated from Prunella vulgaris via the inhibition of NF-kappaB-dependent MMP-9 expression. Food Chem Toxicol 2010; 48(2): 564-71.
[8]
Qu Z, Zhang J, Yang H, et al. Prunella vulgaris L., an Edible and Medicinal Plant, Attenuates Scopolamine-Induced Memory Impairment in Rats. J Agric Food Chem 2017; 65(2): 291-300.
[9]
Li C, Huang Q, Fu X, Yue XJ, Liu RH, You LJ. Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn. Int J Biol Macromol 2015; 75: 298-305.
[10]
Chen ST, Dou J, Temple R, Agarwal R, Wu KM, Walker S. New therapies from old medicines. Nat Biotechnol 2008; 26(10): 1077-83.
[11]
Jung YB, Roh KJ, Jung JA, et al. Effect of SKI 306X, a new herbal anti-arthritic agent, in patients with osteoarthritis of the knee: a double-blind placebo controlled study. Am J Chin Med 2001; 29(3-4): 485-91.
[12]
Yue R, Shan L, Yang X, Zhang W. Approaches to target profiling of natural products. Curr Med Chem 2012; 19(22): 3841-55.
[13]
Oh C, Price J, Brindley MA, et al. Inhibition of HIV-1 infection by aqueous extracts of Prunella vulgaris L. Virol J 2011; 8: 188.
[14]
Brindley MA, Widrlechner MP, McCoy JA, et al. Inhibition of lentivirus replication by aqueous extracts of Prunella vulgaris. Virol J 2009; 6: 8.
[15]
Kajima H, Ogura H. Triterpenoids from Prunella vulgaris. Phytochemistry 1986; 25: 729-33.
[16]
Kajima H, Tominga H, Sato S. Pentacyclic triterpenoids from Prunella vulgaris. Phytochemistry 1987; 26: 1107-11.
[17]
Zhang YJ, Yang CR. Two New Ursane Glycosides from Prunella Vulgaris in France. Yunnan Institute of Botany 1995; 17: 468-72.
[18]
Meng G, Zhang KJ, Zhang MZ. Study on chemical constituents and anticancer activity of Prunella vulgaris L. J Northwest Pharm Univ 2007; 22: 211-3.
[19]
Gu XJ, Li YB, Li P, Qian SH, Duan JA. Studies on chemical constituents of Prunella vulgaris. Zhongguo Zhongyao Zazhi 2007; 32(10): 923-6.
[20]
Wang ZJ, Zhao YY, Tu GZ. Studies on the chemical constituents from Prunella vulgaris. Yao Xue Xue Bao 1999; 34: 679-81.
[21]
Tian J, Xiao ZY, Chen YY. Structure Identification of Vulgarsaponin A. Yao Xue Xue Bao 2000; 35: 29-31.
[22]
Kojima H, Sato N, Hatano A. Sterol glucosides from Prunella vulgaris. Phytochemistry 1990; 29: 2351-5.
[23]
Dmitruk SI, Dmitruk SE, Berezovskaya TP. Flavonoids of Prunella vulgaris. Khim Prir Soe Din 1987; 23: 449-50.
[24]
Meng ZM, He LW. Studies on constituents of Prunella vulgaris L. Zhongguo Yaoke Daxue Xuebao 1995; 26: 329-31.
[25]
Kajima H, Tominga H, Sato S. Two novel hexa2cyclic triterpenoids from Prunella vulgaris. Phytochemistry 1988; 27: 2921-5.
[26]
Dmitruk SI. Coumarins of Prunella vulgaris. Chem Nat Compd 1986; 22: 480.
[27]
Wang ZJ, Zhao YY, Wang B. Depsides from Prunella vulgaris. China Acad J Elect Publ House 2001; 17: 157-61.
[28]
Jain M, Saxena VK. Chemical examination of the fat from the leaves of Brunella vulgaris. J Inst Chem (India) 1984; 56: 133.
[29]
Qin R, Lu J. Advances in studies on chemical constituents and pharmacological effects of Prunella vulgaris. Guide China Mede 2012; 10: 435-6.
[30]
Xu HX, Lee SH, Lee SF, White RL, Blay J. Isolation and characterization of an anti-HSV polysaccharide from Prunella vulgaris. Antiviral Res 1999; 44(1): 43-54.
[31]
Bai Y, Xia B, Xie W, et al. Phytochemistry and pharmacological activities of the genus Prunella. Food Chem 2016; 204: 483-96.
[32]
Xu HY, Jin Y. Study on anti-tumor constituents of Spica Prunellae and their anti-tumor activity. Tradit Chin Drug Res Clin Pharmacol 2015; 26: 351-5.
[33]
Chao L, Qiang H, Jie X, et al. Preparation of Prunella vulgaris polysaccharide-zinc complex and its antiproliferative activity in HepG2 cells. Int J Biol Macromol 2016; 91: 671-9.
[34]
Yin DT, Lei M, Xu J, et al. The Chinese herb Prunella vulgaris promotes apoptosis in human well-differentiated thyroid carcinoma cells via the B-cell lymphoma-2/Bcl-2-associated X protein/caspase-3 signaling pathway. Oncol Lett 2017; 14(2): 1309-14.
[35]
Shen YF, Ding QX, Song LB. Experimental study on inhibition of Prunella vulgaris l. forthree kinds of tumor cells. J N Chin Med 2015; 47: 273-5.
[36]
Wu XM, Xu YP. Effect of Prunella vulgaris L. on proliferation and apoptosis of cholangiocarcinoma cells. Zhejiang Zhong Yi Yao Dai Za Zhi 2017; 52: 227-8.
[37]
Zhou YF, Fan PH. Research Progress of Immunomodulatory Effect of Traditional Chinese Medicine. Lishizhen Medicine and Materia Medica Research 2017; 28: 204-7.
[38]
Yan D, Xie JC, Zhou YM. Substitutability of Prunella vulgaris L Stem Leaf and Ear Based on HPLC-ESI-MSn Analysis and Anti-Inflammatory and Antioxidant Activity. China Pharm J 2016; 51: 792-7.
[39]
Cai S P, Yang Y, Wu R. Pharmacodynamics of Prunella vulgaris and Gel against Herpes Simplex Virus. World Science and Techology/ Modernization of Traditional Chinese Medicine and Materia Medica 2017; 19: 247-53.
[40]
Tan JB, Zhao M, Yang XF. Protetive Effect of Prunella Spica on Oxidative Stress Injury. Zhongguo Shiyan Fangjixue Zazhi 2016; 22: 89-94.
[41]
Feng L, Jia X, Zhu MM, Chen Y, Shi F. Antioxidant activities of total phenols of Prunella vulgaris L. in vitro and in tumor-bearing mice. Molecules 2010; 15(12): 9145-56.
[42]
Shayi-Buzhati M, Chen C, Wang M, Wang J, Zhang XY. Protection of pyrazole-lipopolysaccharide induced liver injury in mice by Prunella vulgaris L. Shipin Anquan Zhiliang Jiance Xuebao 2016; 7: 2334-6.
[43]
Liu H, Zhong YJ, Wu D. Inhibitory Effect of Prunella vulgaris L. Flavonoids on Osteoporosis in Ovariectomized Rats. Xiandai Shipin Keji 2014; 30(8): 6-11.
[44]
Liu YM, Ni YC, Li HB. Antidepressant Effect of the Water Extracts from Prunella vulgaris L. Zhongyao Xinyao Yu Linchuang Yaoli 2017; 440-4.
[45]
Kwon SS, Kwon JY, Park YW, Kim YH, Lim JB. HbA1c for diagnosis and prognosis of gestational diabetes mellitus. Diabetes Res Clin Pract 2015; 110(1): 38-43.
[46]
Rajanandh MG, Kosey S, Prathiksha G. Assessment of antioxidant supplementation on the neuropathic pain score and quality of life in diabetic neuropathy patients - a randomized controlled study. Pharmacol Rep 2014; 66(1): 44-8.
[47]
Arulselvan P, Subramanian SP. Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultra structural changes of pancreatic beta-cells in experimental diabetes in rats. Chem Biol Interact 2007; 165(2): 155-64.
[48]
Punitha IS, Rajendran K, Shirwaikar A, Shirwaikar A. Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin-nicotinamide induced diabetic rats. Evid Based Complement Alternat Med 2005; 2(3): 375-81.
[49]
Manonmani G, Bhavapriya V, Kalpana S, Govindasamy S, Apparanantham T. Antioxidant activity of Cassia fistula (Linn.) flowers in alloxan induced diabetic rats. J Ethnopharmacol 2005; 97(1): 39-42.
[50]
Raafat K, Wurglics M, Schubert-Zsilavecz M. Prunella vulgaris L. active components and their hypoglycemic and antinociceptive effects in alloxan-induced diabetic mice. Biomed Pharmacother 2016; 84: 1008-18.
[51]
Li YL. Study on Antihypertensive Effect of Prunella vulgaris Extract on Spontaneously Hypertensive Rats. Chinese and Foreign Medical Research 2012; 180: 147.
[52]
Kim HI, Quan FS, Kim JE, et al. Inhibition of estrogen signaling through depletion of estrogen receptor alpha by ursolic acid and betulinic acid from Prunella vulgaris var. lilacina. Biochem Biophys Res Commun 2014; 451(2): 282-7.
[53]
Psotova J, Svobodova A, Kolarova H, Walterova D. Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes. J Photochem Photobiol B 2006; 84(3): 167-74.
[54]
Psotová J, Chlopcíková S, Miketová P, Simánek V. Cytoprotectivity of Prunella vulgaris on doxorubicin-treated rat cardiomyocytes. Fitoterapia 2005; 76(6): 556-61.
[55]
Huang R, Zhao M, Yang X, et al. Effects of Prunella vulgaris on the mice immune function. PLoS One 2013; 8(10)e77355
[56]
Jun MS, Kim HS, Kim YM, et al. Ethanol extract of Prunella vulgaris var. lilacina inhibits HMGB1 release by induction of heme oxygenase-1 in LPS-activated RAW 264.7 cells and CLP-induced septic mice. Phytother Res 2012; 26(4): 605-12.
[57]
Kageyama S, Kurokawa M, Shiraki K. Extract of Prunella vulgaris spikes inhibits HIV replication at reverse transcription in vitro and can be absorbed from intestine in vivo. Antivir Chem Chemother 2000; 11(2): 157-64.
[58]
Han EH, Choi JH, Hwang YP, et al. Immunostimulatory activity of aqueous extract isolated from Prunella vulgaris. Food Chem Toxicol 2009; 47(1): 62-9.
[59]
Hwang YJ, Lee EJ, Kim HR, Hwang KA. In vitro antioxidant and anticancer effects of solvent fractions from Prunella vulgaris var. lilacina. BMC Complement Altern Med 2013; 13: 310.
[60]
Shin TY, Kim YK, Kim HM. Inhibition of immediate-type allergic reactions by Prunella vulgaris in a murine model. Immunopharmacol Immunotoxicol 2001; 23(3): 423-35.
[61]
Harput US, Saracoglu I, Ogihara Y. Effects of two Prunella species on lymphocyte proliferation and nitric oxide production. Phytother Res 2006; 20(2): 157-9.
[62]
Haarberg KM, Wymore Brand MJ, Overstreet AM, et al. Orally administered extract from Prunella vulgaris attenuates spontaneous colitis in mdr1a(-/-) mice. World J Gastrointest Pharmacol Ther 2015; 6(4): 223-37.
[63]
Wu H, Gao M, Ha T, Kelley J, Young A, Breuel K. Prunella vulgaris aqueous extract attenuates IL-1β-induced apoptosis and NF-κB activation in INS-1 cells. Exp Ther Med 2012; 3(6): 919-24.
[64]
Zdarilová A, Svobodová A, Simánek V, Ulrichová J. Prunella vulgaris extract and rosmarinic acid suppress lipopolysaccharide-induced alteration in human gingival fibroblasts. Toxicol In Vitro 2009; 23(3): 386-92.
[65]
Raafat K, Wurglics M, Schubert-Zsilavecz M. Prunella vulgaris L. active components and their hypoglycemic and antinociceptive effects in alloxan-induced diabetic mice. Biomed Pharmacother 2016; 84: 1008-18.
[66]
Zhang M, Hwang E, Lin P, Gao W, Ngo HTT, Yi TH. Prunella vulgaris L. Exerts a Protective Effect Against Extrinsic Aging Through NF-κB, MAPKs, AP-1, and TGF-β/Smad Signaling Pathways in UVB-Aged Normal Human Dermal Fibroblasts. Rejuvenation Res 2018; 21(4): 313-22.
[67]
Xia N, Bollinger L, Steinkamp-Fenske K, Förstermann U, Li H. Prunella vulgaris L. Upregulates eNOS expression in human endothelial cells. Am J Chin Med 2010; 38(3): 599-611.
[68]
Qu Z, Zhang J, Yang H, et al. Prunella vulgaris L., an Edible and Medicinal Plant, Attenuates Scopolamine-Induced Memory Impairment in Rats. J Agric Food Chem 2017; 65(2): 291-300.
[69]
Hwang SM, Lee YJ, Yoon JJ, et al. Prunella vulgaris suppresses HG-induced vascular inflammation via Nrf2/HO-1/eNOS activation. Int J Mol Sci 2012; 13(1): 1258-68.
[70]
Huang N, Hauck C, Yum MY, et al. Rosmarinic acid in Prunella vulgaris ethanol extract inhibits lipopolysaccharide-induced prostaglandin E2 and nitric oxide in RAW 264.7 mouse macrophages. J Agric Food Chem 2009; 57(22): 10579-89.
[71]
Park SJ, Ahn YJ, Lee HE, Hong E, Ryu JH. Standardized Prunella vulgaris var. lilacina Extract Enhances Cognitive Performance in Normal Naive Mice. Phytother Res 2015; 29(11): 1814-21.
[72]
Park SJ, Kim DH, Lee IK, et al. The ameliorating effect of the extract of the flower of Prunella vulgaris var. lilacina on drug-induced memory impairments in mice. Food Chem Toxicol 2010; 48(6): 1671-6.
[73]
Kim SH, Huang CY, Tsai CY, Lu SY, Chiu CC, Fang K. The aqueous extract of Prunella vulgaris suppresses cell invasion and migration in human liver cancer cells by attenuating matrix metalloproteinases. Am J Chin Med 2012; 40(3): 643-56.
[74]
Zhang Y, But PP, Ooi VE, et al. Chemical properties, mode of action, and in vivo anti-herpes activities of a lignin-carbohydrate complex from Prunella vulgaris. Antiviral Res 2007; 75(3): 242-9.
[75]
Skottová N, Kazdová L, Oliyarnyk O, Vecera R, Sobolová L, Ulrichová J. Phenolics-rich extracts from Silybum marianum and Prunella vulgaris reduce a high-sucrose diet induced oxidative stress in hereditary hypertriglyceridemic rats. Pharmacol Res 2004; 50(2): 123-30.
[76]
Li C, Huang Q, Xiao J, Fu X, You L, Liu RH. Preparation of Prunella vulgaris polysaccharide-zinc complex and its antiproliferative activity in HepG2 cells. Int J Biol Macromol 2016; 91: 671-9.
[77]
Hao J, Ding XL, Yang X, Wu XZ. Prunella vulgaris polysaccharide inhibits growth and migration of breast carcinoma-associated fibroblasts by suppressing expression of basic fibroblast growth factor. Chin J Integr Med 2016.
[http://dx.doi.org/10.1007/s11655-016-2]
[78]
Huang MX, Zou K, He K. The improvement of quality standard about prunellae spica granule. Ningxia Med J 2016; 38: 329-31.
[79]
Yuan JS, Wang PY, Wang J. Curative effect of selfheal granules in treatment of xerophthalmia in menopausal women and its effect on expressions of IL-1β, TNF-α, and ICAM-1 in conjunctiva. Zhongguo Fuyou Baojian 2017; 32: 2675-8.
[80]
Ying ZY. Clinical observation of Xiakucao Granules combined with Thiamazole Tablets in treatment of diffuse goiter with hyperthyroidism. Drugs & Clinic 2016; 31: 70-4.
[81]
Gong XJ, Liu WG, Zhong H. Prunella tablets treatment of benign prostatic hyperplasia in 40 cases. J Pract Tradit Chin Med 2014; 30: 964-5.
[82]
Mo YT, Liu P. Clinical study of prunella combined with glucocorticoids on subacute thyroiditis. Asia-Pacific Traditional Medicine 2015; 11: 119-20.
[83]
Hu Y, Dai XY, Han YY. Effect of Prunella vulgaris Tablets and Adapalene Gel on Acne Vulgaris. Asia-Pacific Traditional Medicine 2014; 10: 108-9.
[84]
Zhong RY. Prunella cream adjuvant treatment of thyroid nodules. Aerosp Med 2014; 368-9.
[85]
Li DJ, Wang Y, Zhao T. Clinical study on Xiakucao Oral Liquid combined with compound betamethasone in treatment of subacute thyroiditis. Drugs & Clinic 2017; 32: 1714-7.
[86]
Li X, Liu W, Niu B. Clinical observation of prunella oral liquid and cefdinir dispersible tablets in treating chronic mastitis. Pharmacol Clin Tradit Chin Med 2017; 33: 190-2.
[87]
Fan ZY, Zhang LL, Mi R. Effect of Xiakucao capsule on Hashimoto thyroiditis and the ultrasonic diagnosis of thyroid morphology before and after treament. J Hebei Med Univ 2017; 38: 446-9.
[88]
Cheung HY, Zhang QF. Enhanced analysis of triterpenes, flavonoids and phenolic compounds in Prunella vulgaris L. by capillary zone electrophoresis with the addition of running buffer modifiers. J Chromatogr A 2008; 1213(2): 231-8.