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Current Pharmaceutical Analysis

Author(s): Palak R. Patel, Nikunj D. Patel, Samir G. Patel, Niranjan S. Kanaki* and Archita J. Patel*

DOI: 10.2174/1573412915666190312161325

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Fingerprint Analysis of Vitex Negundo by HPLC Coupled with Multi-components Analysis

Page: [743 - 751] Pages: 9

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Abstract

Background: Vitex negundo (Nagod) is a very useful medicinal plant growing throughout India. The leaves of Nagod are aromatic, tonic and vermifuge. They are useful in the treatment of many ailments.

Introduction: Both seasonal and geographical variations have been observed in the active constituents in Vitex negundo. A comprehensive and reproducible HPLC method based on HPLC fingerprint analysis was developed for assessing the quality of Vitex negundo.

Methods: Sixteen samples of Nagod collected from different locations and seasons of India were analysed by HPLC and chromatograms were recorded for each of them using PDA detector and 10 peaks were considered for further data analysis. The data were then treated for PCA and cluster analysis using Minitab software.

Results: PCA and HCA analysis were used in determining the variability in the leaves of Nagod collected from different places and seasons.

Conclusion: The method was useful for discriminating the location of plant within or outside Gujarat but was unable to display any effective seasonal variation in collected samples.

Keywords: HPLC, fingerprinting, Vitex negundo, PCA, HCA, quality control.

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[1]
Yongyu, Z.; Shujun, S.; Jianye, D.; Wenyu, W.; Huijuan, C.; Jianbing, W.; Xiaojun, G. Quality control method for herbal medicine-chemical fingerprint analysis; Quality Control of Herbal Medicines and Related Area, 2011, pp. 171-194.
[http://dx.doi.org/10.5772/23962]
[2]
Chen, F.F.; Qi, H.Y.; Shi, Y.P. Fingerprint analysis of Codonopsis Radix by HPLC coupled with chemometrics analysis. Chin. Herb. Med., 2013, 5(4), 307-312.
[http://dx.doi.org/10.1016/S1674-6384(13)60046-5]
[3]
Li, Y.; Wu, T.; Zhu, J.; Wan, L.; Yu, Q.; Li, X.; Cheng, Z.; Guo, C. Combinative method using HPLC fingerprint and quantitative analyses for quality consistency evaluation of an herbal medicinal preparation produced by different manufacturers. J. Pharm. Biomed. Anal., 2010, 52(4), 597-602.
[http://dx.doi.org/10.1016/j.jpba.2010.01.018] [PMID: 20138726]
[4]
Liang, Y.; Xie, P.; Chau, F. Chromatographic fingerprinting and related chemometric techniques for quality control of traditional Chinese medicines. J. Sep. Sci., 2010, 33(3), 410-421.
[http://dx.doi.org/10.1002/jssc.200900653] [PMID: 20099260]
[5]
Kulkarni, K.M.; Patil, L.S.; Khanvilkar, V.V.; Kadam, V.J. Fingerprinting techniques in herbal standardization. Indo American J. Pharm. Res., 2014, 4, 1049-1062.
[6]
Lin, S.; Gao, W.; Tian, Z.; Yang, C.; Lu, L.; Mergny, J.L.; Leung, C.H.; Ma, D.L. Luminescence switch-on detection of protein tyrosine kinase-7 using a G-quadruplex-selective probe. Chem. Sci. (Camb.), 2015, 6(7), 4284-4290.
[http://dx.doi.org/10.1039/C5SC01320H] [PMID: 29218197]
[7]
Collot, M.; Fam, T.K.; Ashokkumar, P.; Faklaris, O.; Galli, T.; Danglot, L.; Klymchenko, A.S. Ultrabright and fluorogenic probes for multicolor imaging and tracking of lipid droplets in cells and tissues. J. Am. Chem. Soc., 2018, 140(16), 5401-5411.
[http://dx.doi.org/10.1021/jacs.7b12817] [PMID: 29446627]
[8]
Wang, M.; Mao, Z.; Kang, T.S.; Wong, C.Y.; Mergny, J.L.; Leung, C.H.; Ma, D.L. Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior. Chem. Sci. (Camb.), 2016, 7(4), 2516-2523.
[http://dx.doi.org/10.1039/C6SC00001K] [PMID: 28660021]
[9]
Bansal, A.; Chhabra, V.; Rawal, R.K.; Sharma, S. Chemometrics: A new scenario in herbal drug standardization. J. Pharm. Anal., 2014, 4(4), 223-233.
[http://dx.doi.org/10.1016/j.jpha.2013.12.001] [PMID: 29403886]
[10]
Tian, R.T.; Xie, P.S.; Liu, H.P. Evaluation of traditional Chinese herbal medicine: Chaihu (Bupleuri Radix) by both high-performance liquid chromatographic and high-performance thin-layer chromatographic fingerprint and chemometric analysis. J. Chromatogr. A, 2009, 1216(11), 2150-2155.
[http://dx.doi.org/10.1016/j.chroma.2008.10.127] [PMID: 19084233]
[11]
Xie, B.; Gong, T.; Tang, M.; Mi, D.; Zhang, X.; Liu, J.; Zhang, Z. An approach based on HPLC-fingerprint and chemometrics to quality consistency evaluation of Liuwei Dihuang Pills produced by different manufacturers. J. Pharm. Biomed. Anal., 2008, 48(4), 1261-1266.
[http://dx.doi.org/10.1016/j.jpba.2008.09.011] [PMID: 18930621]
[12]
Feng, X.; Kong, W.; Wei, J.; Ou-Yang, Z.; Yang, M. HPLC fingerprint analysis combined with chemometrics for pattern recognition of ginger. Pharm. Biol., 2014, 52(3), 362-367.
[http://dx.doi.org/10.3109/13880209.2013.837493] [PMID: 24171804]
[13]
Singh, P.; Mishra, G.; Srivastava, S.; Sangeeta, K.; Khosa, R. Phytopharmacological review of Vitex negundo (Sambhalu). Pharmacologyonline, 2011, 2, 1355-1385.
[14]
Shailajan, S.; Yeragi, M.; Matani, A.; Gurjar, D.; Singh, D.; Tiwari, B. Impact of regional variation on β-sitosterol content in Vitex nigundo Linn. fruits: Evaluation using HPTLC. J. Adv. Sci. Res., 2014, 5(2), 46-49.
[15]
Desai, S. Development and validation of HPLC methods for marker compounds and bioactive as per ICH guidelinesPhD Thesis. SNDT Women’s University, Mumbai, India, 2014. May
[16]
Britto, J.; Sujin, M. HPLC analysis of vitexin and fingerprint of Vitex negundo L. Int. J. Pharm. Pharm. Sci., 2012, 4(2), 138-141.
[17]
Sun, S.; Liu, H.; Xu, S.; Yan, Y.; Xie, P. Quality analysis of commercial samples of Ziziphi spinosae semen (suanzaoren) by means of chromatographic fingerprinting assisted by principal component analysis. J. Pharm. Anal., 2014, 4(3), 217-222.
[http://dx.doi.org/10.1016/j.jpha.2014.01.003] [PMID: 29403885]
[18]
Tian, L.; Zhao, Y.; Zhou, X.; Chen, H.-G.; Zhao, C.; Gong, X.-J. 2013, 1-15.
[19]
Lucio-Gutiérrez, J.R.; Garza-Juárez, A.; Coello, J.; Maspoch, S.; Salazar-Cavazos, M.L.; Salazar-Aranda, R.; Waksman de Torres, N. Multi-wavelength high-performance liquid chromatographic fingerprints and chemometrics to predict the antioxidant activity of Turnera diffusa as part of its quality control. J. Chromatogr. A, 2012, 1235, 68-76.
[http://dx.doi.org/10.1016/j.chroma.2012.02.042] [PMID: 22405537]
[20]
Zhu, J.; Fan, X.; Cheng, Y.; Agarwal, R.; Moore, C.M.; Chen, S.T.; Tong, W. Chemometric analysis for identification of botanical raw materials for pharmaceutical use: a case study using Panax notoginseng. PLoS One, 2014, 9(1) e87462
[http://dx.doi.org/10.1371/journal.pone.0087462] [PMID: 24498109]
[21]
Patel, N.; Kanaki, N. Fingerprint analysis of Shankhpushpi for species discrimina-tion by HPLC coupled with chemometric methods. J. Liq. Chrom. Relat. Tech., 2020.
[22]
Chen, Y.; Zhu, S.B.; Xie, M.Y.; Nie, S.P.; Liu, W.; Li, C.; Gong, X.F.; Wang, Y.X. Quality control and original discrimination of Ganoderma lucidum based on high-performance liquid chromatographic fingerprints and combined chemometrics methods. Anal. Chim. Acta, 2008, 623(2), 146-156.
[http://dx.doi.org/10.1016/j.aca.2008.06.018] [PMID: 18620918]
[23]
Harmonized Triparticle Guideline, I.C.H.. Validation of Analytical Procedures: Text and Methodology Q2 (R1International Conference on Harmonization; Geneva, Switzerland, 2005.