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Current Drug Discovery Technologies

Author(s): Lokesh Adhikari, Rupali Kotiyal, Mukesh Pandey, Meenakshi Bharkatiya, Ajay Sematy and Mona Semalty*

DOI: 10.2174/1570163815666180807100456

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Effect of Geographical Location and Type of Extract on Total Phenol/Flavon Contents and Antioxidant Activity of Different Fruits Extracts of Withania somnifera

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Abstract

Background: Withania somnifera (family solanaceae) is a well-investigated medicinal plant which is also called Indian ginseng due to its wide spectrum of medicinal properties. The contents and activity of the plant may vary depending on the habitat and part of the plant and the solvent used for extraction. The present study deals with the comparative chemical analysis and in vitro antioxidant activity of methanolic fruits extracts and its subfractions (in ethyl acetate, butanol and water) of W. somnifera collected from two different geographical locations.

Methods: In the present study, Withania somnifera fruits were collected from two different geographical locations (Uttarakhand and Rajasthan). The different fruit extracts were prepared and studied for total phenolic contents and total flavone contents. The in vitro antioxidant activity was assessed by DPPH free radical scavenging assay and peroxide scavenging assay.

Results: Methanol extract of W. somnifera Uttarakhand and ethyl acetate subfraction of W. somnifera Rajasthan showed the highest amount of Total Phenolic Contents (TPC). In W. somnifera Uttarakhand, ethyl acetate extract showed the highest amount of Total flavonoids while in W. somnifera Rajasthan, methanol extract was found to be the richest in flavonoids. Methanolic extract of W. somnifera Uttarakhand showed the highest free radical scavenging activity while in W. somnifera Rajasthan, the highest antioxidant activity was shown by the methanolic extract followed by butanolic extract, water extract and then ethyl acetate. In the peroxide scavenging assay of antioxidant activity, water extract of W. somnifera Uttarakhand showed the highest activity, while in W. somnifera Rajasthan, ethyl acetate extract showed highest scavenging activity.

Conclusion: It was concluded that the geographical location exerts a vital effect on the presence of active constituents and also on the antioxidant potential of W. somnifera.

Keywords: Phenolic contents, antioxidant activity, DPPH, geographical location, Uttarakhand, Rajasthan, Withania.

Graphical Abstract

[1]
Mishra LC, Singh BB, Dagenais S. Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): A review. Altern Med Rev 2000; 5(4): 334-46.
[PMID: 10956379]
[2]
Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. New Delhi: Central Drug Research Institute 1998; p. 6.
[3]
Umadevi M, Rajeswari R, Sharmila RC, et al. Traditional and medicinal uses of Withania somnifera. Pharma Innovation 2012; 9(1): 103-4.
[4]
Kaur N, Niazi J, Bains R. A review on pharmacological profile of Withania somnifera (Ashwagandha). Res Rev J Bot Sci 2013.
[5]
Uddin Q, Samiulla L, Singh VK, Jamil SS. Phytochemical and pharmacological profile of Withania somnifera Dunal: A review. J Appl Pharm Sci 2012; 2(1): 170-5.
[6]
Khare CP. Indian Medicinal Plants-An Illustrated Dictionary. (India), New Delhi: First Indian Reprint, Springer 2007; pp. 717-8.
[http://dx.doi.org/10.1007/978-0-387-70638-2]
[7]
Bhattacharya SK, Satyan KS, Ghosal S. Antioxidant activity of glycowithanolides from Withania somnifera. Indian J Exp Biol 1997; 35(3): 236-9.
[PMID: 9332168]
[8]
Davis L, Kuttan G. Immunomodulatory activity of Withania somnifera. J Ethnopharmacol 2000; 71(1-2): 193-200.
[http://dx.doi.org/10.1016/S0378-8741(99)00206-8] [PMID: 10904163]
[9]
Singh G, Sharma PK, Dudhe R, Singh S. Biological activities of Withania somnifera. Ann Biol Res 2010; 1(3): 56-63.
[10]
Jain R, Kachhwaha S, Kothari SLA. Phytochemistry, pharmacology, and biotechnology of Withania somnifera and Withania coagulans. J Med Plants Res 2012; 6(41): 5388-99.
[http://dx.doi.org/10.5897/JMPR12.704]
[11]
Gupta A, Singh S. Evaluation of anti-inflammatory effect of Withania somnifera root on collagen-induced arthritis in rats. Pharm Biol 2014; 52(3): 308-20.
[http://dx.doi.org/10.3109/13880209.2013.835325] [PMID: 24188460]
[12]
Udayakumar R, Kasthurirengan S, Vasudevan A, et al. Antioxidant effect of dietary supplement Withania somnifera L. reduce blood glucose levels in alloxan-induced diabetic rats. Plant Foods Hum Nutr 2010; 65(2): 91-8.
[http://dx.doi.org/10.1007/s11130-009-0146-8] [PMID: 20186490]
[13]
Mohanty I, Arya DS, Dinda A, Talwar KK, Joshi S, Gupta SK. Mechanisms of cardioprotective effect of Withania somnifera in experimentally induced myocardial infarction. Basic Clin Pharmacol Toxicol 2004; 94(4): 184-90.
[http://dx.doi.org/10.1111/j.1742-7843.2004.pto940405.x] [PMID: 15078343]
[14]
Naidu PS, Singh A, Kulkarni SK. Effect of Withania somnifera root extract on haloperidol-induced orofacial dyskinesia: possible mechanisms of action. J Med Food 2003; 6(2): 107-14.
[http://dx.doi.org/10.1089/109662003322233503] [PMID: 12935321]
[15]
Yu Y, Hamza A, Zhang T, et al. Withaferin A targets heat shock protein 90 in pancreatic cancer cells. Biochem Pharmacol 2010; 79(4): 542-51.
[http://dx.doi.org/10.1016/j.bcp.2009.09.017] [PMID: 19769945]
[16]
Ichikawa H. Withanolides potentiate apoptosis, inhibit invasion, and abolish osteoclastogenesis through suppression of nuclear factor-KB (NF-KB) activation and NF-KB-regulated gene expression. Mol Cancer Thera 2006; 5(6)
[17]
Mohammad S. Ismail MdH, Anwar SF, Akhter, Sadika, Haque MdA, Ahmed BM. In vitro antioxidant and free radical scavenging activity of Withania somnifera Root. Iosr J Pharm 2013; 3(2): 38-47.
[18]
Demiray S, Pintado ME, Castro PML. Evaluation of phenolic profiles and antioxidant activities of turkish medicinal plants: Tilia argentea, crataegi folium leaves and polygonum bistorta roots. W Academy of Sci Eng Tech 2009; 54: 312-7.
[19]
Jiao H, Wang SY. Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. J Agric Food Chem 2000; 48(11): 5672-6.
[http://dx.doi.org/10.1021/jf000765q] [PMID: 11087537]
[20]
Sematly M, Sematly A, Rawat BS, Singh D, Joshi GP, Rawat MSM. In vitro antioxidant activity of roots of Urtica dioica (Nettle). Indian Drugs 2010; 47: 55-6.
[21]
Jayaprakasha GK, Singh RP, Sakariah KK. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem 2001; 73(3): 285-90.
[http://dx.doi.org/10.1016/S0308-8146(00)00298-3]
[22]
Pal A, Kumar M, Saharan V, Bhushan B. Antioxidant and Free radical Scavenging activity of Ashwagandha (Withania somnifera L.)Leaves. J Global Bioscci 2015; 4(1): 1127-37.
[23]
Braca A, De Tommasi N, Di Bari L, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia tarapotensis. J Nat Prod 2001; 64(7): 892-5.
[http://dx.doi.org/10.1021/np0100845] [PMID: 11473417]
[24]
Dhanani T, Shah S, Gajbhiye NA, Kumar S. Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arab J Chem 2017; 10(1): S1193-9.
[http://dx.doi.org/10.1016/j.arabjc.2013.02.015]
[25]
Alam N, Hossain M, Mottalib MA, Sulaiman SA, Gan SH, Khalil MI. Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities. BMC Complement Altern Med 2012; 12: 175.
[http://dx.doi.org/10.1186/1472-6882-12-175] [PMID: 23039061]
[26]
Mehrotra V, Mehrotra S, Kirar V, et al. Antioxidant and antimicrobial activities of aqueous extract of Withania somnifera against methicillin-resistant Staphylococcus aureus. J Micro Biotech Res 2011; 1(1): 40-5.
[27]
Andarwulan N, Batari R, Sandrasari DA, Bolling B, Wijaya H. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem 2010; 121(4): 1231-5.
[http://dx.doi.org/10.1016/j.foodchem.2010.01.033] [PMID: 28814820]
[28]
Ghasemi Pirbalouti A, Siahpoosh A, Setayesh M, Craker L. Antioxidant activity, total phenolic and flavonoid contents of some medicinal and aromatic plants used as herbal teas and condiments in Iran. J Med Food 2014; 17(10): 1151-7.
[http://dx.doi.org/10.1089/jmf.2013.0057] [PMID: 25084312]
[29]
Senthil K, Thirugnanasambantham P, Oh TJ, Kim SH, Choi HK, Choi HK. Free radical scavenging activity and comparative metabolic profiling of in vitro cultured and field grown Withania somnifera roots. PloS One 2015; 10(4): e0123360.30..
[30]
Senthil K, Thirugnanasambantham P, Oh TJ, Kim SH, Choi HK. Free radical scavenging activity and comparative metabolic profiling of in vitro cultured and field grown Withania somnifera roots. PLoS One 2015; 10(4) e0123360
[http://dx.doi.org/10.1371/journal.pone.0123360] [PMID: 25874568]
[31]
Dar NJ, Hamid A, Ahmad M. Pharmacologic overview of Withania somnifera, the Indian Ginseng. Cell Mol Life Sci 2015; 72(23): 4445-60.
[http://dx.doi.org/10.1007/s00018-015-2012-1] [PMID: 26306935]
[32]
Kumar S, Yadav A, Yadav M, Yadav JP. Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloe vera (L.) Burm.f. BMC Res Notes 2017; 10(1): 60.
[http://dx.doi.org/10.1186/s13104-017-2385-3] [PMID: 28118858]
[33]
Sanders TH. Peanut triacylglycerols: effect of season and production location. J Am Oil Chem Soc 1982; 59: 346-51.
[http://dx.doi.org/10.1007/BF02541017]
[34]
Kumar S, Budhwar L, Yadav A, Yadav M, Yadav JP. Phytochemical screening and antibacterial activity of Aloe vera collected from different climatic regions of India. Nat Prod J 2016; 6: 73-82.
[http://dx.doi.org/10.2174/2210315506666151208213012]
[35]
Anwar F, Manzoor M, Bajwa J. Antioxidant activity of solvent extracts of strawberry using various antioxidant assay. Pak J Anal Chem 2004; 5: 28-37.