Generic placeholder image

Current Bioactive Compounds

Author(s): Chaima Mouffouk, Leila Hambaba, Hamada Haba*, Soumia Mouffouk and Chawki Bensouici

DOI: 10.2174/1573407214666180730102338

DownloadDownload PDF Flyer Cite As
Evaluation of Cytotoxic Effect, Anti-Cholinesterase, Hemolytic and Antibacterial activities of the Species Scabiosa stellata L.

Page: [72 - 79] Pages: 8

  • * (Excluding Mailing and Handling)

Explore Articles
About Journal
For Authors
For Editors
For Reviewers

Abstract

Objectives: In this study, cytotoxic effect, anticholinesterase, hemolytic and antibacterial activities of crude extracts (petroleum ether, ethyl acetate and n-butanol) obtained from the plant Scabiosa stellata L. were evaluated.

Methods: The cytotoxicity of extracts was tested by Brine shrimp lethality method; the acetylcholinesterase inhibitory activity was performed using Ellman's colorimetric method and the hemolytic activity was assessed by spectrophotometric method towards human erythrocytes. Furthermore, the antibacterial activity was estimated by agar disk diffusion assay against ten bacterial strains.

Results: The phytochemical screening of the extracts revealed the presence of several types of secondary metabolites. A significant cytotoxic effect was observed for the n-butanolic extract with 57.2 ± 0.2 % of mortality at 80 μg/mL, the ethyl acetate extract had a moderate anticholinesterase activity at 200 μg/mL. The hemolytic assay exhibited that n-butanolic and ethyl acetate extracts induce hemolysis in dose-dependent manner with values of EC50 at 37.3 ± 0.5 and 106.6 ± 0.3 μg/mL, respectively. All the crude extracts showed antibacterial activity against most tested strains, with zones of inhibition ranging from 9 to 20 mm.

Conclusion: The results indicate that the extracts obtained from S. stellata can be an important source of therapeutic agents against pathological damage due to free radicals inducing neurodegenerative and infectious diseases, while n-butanolic extract could be used as a good source of alternative natural antiproliferative compounds.

Keywords: Cytotoxicity, anticholinesterase activity, hemolytic activity, antibacterial activity, phytochemical screening, neurodegenerative diseases.

Graphical Abstract

[1]
Nobili, S.; Lippi, D.; Witort, E.; Donnini, M.; Bausi, L.; Mini, E.; Capaccioli, S. Natural compounds for cancer treatment and prevention. Pharmacol. Res., 2009, 59(6), 365-378.
[http://dx.doi.org/10.1016/j.phrs.2009.01.017] [PMID: 19429468]
[2]
Uttara, B.; Singh, A.V.; Zamboni, P.; Mahajan, R.T. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr. Neuropharmacol., 2009, 7, 65-74.
[http://dx.doi.org/10.2174/157015909787602823]
[3]
Mohammedi, Z.; Atik, F. Hemolytic activity of different herbal extracts used in Algeria. Int. J. Pharm. Sci. Res., 2014, 5, 8495-8504.
[4]
Carlson, S.E.; Linder, H.P.; Donoghue, M.J. The historical biogeography of Scabiosa (Dipsacaceae): Implications for old world plant disjunctions. J. Biogeogr., 2012, 39, 1086-1100.
[http://dx.doi.org/10.1111/j.1365-2699.2011.02669.x]
[5]
Bonet, M.À.; Parada, M.; Selga, A.; Vallès, J. Studies on pharmaceutical ethnobotany in the regions of L’Alt Empordà and Les Guilleries (Catalonia, Iberian Peninsula). J. Ethnopharmacol., 1999, 68(1-3), 145-168.
[http://dx.doi.org/10.1016/S0378-8741(99)00083-5] [PMID: 10624874]
[6]
Bussmann, R.W.; Glenn, A.; Meyer, K.; Kuhlman, A.; Townesmith, A. Herbal mixtures in traditional medicine in Northern Peru. J. Ethnobiol. Ethnomed., 2010, 6, 10.
[http://dx.doi.org/10.1186/1746-4269-6-10] [PMID: 20226092]
[7]
Bonet, M.À.; Vallès, J. Ethnobotany of Montseny biosphere reserve (Catalonia, Iberian Peninsula): plants used in veterinary medicine. J. Ethnopharmacol., 2007, 110(1), 130-147.
[http://dx.doi.org/10.1016/j.jep.2006.09.016] [PMID: 17059874]
[8]
Hotta, M.; Ogata, K.; Nitta, A.; Hoshikawa, K.; Yanagi, M.; Yamazaki, K. Useful plants of the world, 1st ed; Heibonsha LTD: Tokyo, 1989.
[9]
Marhuenda-Requena, E.; Saenz-Rodriguez, M.T.; Garcia-Gimenez, M.D. A contribution to the pharmacodynamic study of Scabiosa atropurpurea L.I. Analgesic and antipyretic activity. Plant. Med. Phytoter., 1987, 21, 47-55.
[10]
Kupeli, E.; Orhan, I.; Yesilada, E. Evaluation of some plants used in Turkish folk medicine for their anti-inflammatory and antinociceptive activities. Pharm. Biol., 2007, 45, 547-555.
[http://dx.doi.org/10.1080/13880200701498895]
[11]
Hlila, M.B.; Mosbah, H.; Majouli, K.; Msaada, K.; Jannet, H.B.; Aouni, M.; Selmi, B. α-Glucosidase inhibition by Tunisian Scabiosa arenaria Forssk. extracts. Int. J. Biol. Macromol., 2015, 77, 383-389.
[http://dx.doi.org/10.1016/j.ijbiomac.2015.03.035] [PMID: 25841374]
[12]
Hlila, M.B.; Mosbah, H.; Mssada, K.; Jannet, H.B.; Aouni, M.; Selmi, B. Acetylcholinesterase inhibitory and antioxidant properties of roots extracts from the Tunisian scabiosa arenaria Forssk. Ind. Crops Prod., 2015, 67, 62-69.
[http://dx.doi.org/10.1016/j.indcrop.2015.01.009]
[13]
Besbes, M.; Omri, A.; Cheraif, I.; Daami, M.; Jannet, H.B.; Mastouri, M.; Aouni, M.; Selmi, B. Chemical composition and antimicrobial activity of essential oils from Scabiosa arenaria Forssk: growing wild in Tunisia. Chem. Biodivers., 2012, 9(4), 829-839.
[http://dx.doi.org/10.1002/cbdv.201100191] [PMID: 22492499]
[14]
Ma, J.N.; Bolraa, S.; Ji, M.; He, Q.Q.; Ma, C.M. Quantification and antioxidant and anti-HCV activities of the constituents from the inflorescences of Scabiosa comosa and S. tschilliensis. Nat. Prod. Res., 2016, 30(5), 590-594.
[http://dx.doi.org/10.1080/14786419.2015.1027701] [PMID: 25835468]
[15]
Wang, J.; Liu, K.; Xu, D.; Wang, Q.; Bi, K.; Song, Y.; Li, J.; Zhang, L. Rapid micropropagation system in vitro and antioxidant activity of Scabiosa tschiliensis Grunning. J. Plant Growth Regul., 2013, 69, 305-310.
[http://dx.doi.org/10.1007/s10725-012-9765-4]
[16]
Quezel, P.; Santa, S. Nouvelle flore de l’Algérie et des régions désertiques méridionales; Editions du Centre National de la Recherche Scientifique: Paris, 1963.
[17]
Boulos, L. Endemic flora of the middle east and North Africa. Review in ecology: Desert Conservation and Development; Metropole: Cairo, 1997, pp. 229-260.
[18]
Wannes, W.A.; Marzouk, B. Research progress of Tunisian medicinal plants used for acute diabetes. J. Acute Dis., 2016, 5, 357-363.
[http://dx.doi.org/10.1016/j.joad.2016.08.001]
[19]
Bammi, J.; Douira, A. Medicinal plants in the forest of Achach (Central plateau, Morocco). J. Acta Bot. Malacit., 2002, 27, 131-145.
[20]
Farnsworth, N.R. Biological and phytochemical screening of plants. J. Pharm. Sci., 1966, 55(3), 225-276.
[http://dx.doi.org/10.1002/jps.2600550302] [PMID: 5335471]
[21]
Meyer, B.N.; Ferrigni, N.R.; Putnam, J.E.; Jacobsen, L.B.; Nichols, D.E.; McLaughlin, J.L. Brine shrimp: a convenient general bioassay for active plant constituents. J. Med. Plants Res., 1982, 45, 31-34.
[http://dx.doi.org/10.1055/s-2007-971236]
[22]
Ellman, G.L.; Courtney, K.D.; Andres, V., Jr; Feather-Stone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol., 1961, 7, 88-95.
[http://dx.doi.org/10.1016/0006-2952(61)90145-9] [PMID: 13726518]
[23]
Kumar, G.; Karthik, L.; Rao, K.V.B. Hemolytic activity of Indian medicinal plants towards human erythrocytes: An in vitro study. Elixir. Appl. Botany., 2011, 40, 5534-5537.
[24]
Medini, F.; Fellah, H.; Ksouri, R.; Abdelly, C. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. J. Taibah. Univ. Sci., 2014, 8, 216-224.
[http://dx.doi.org/10.1016/j.jtusci.2014.01.003]
[25]
Bahadori, M.B.; Valizadeh, H.; Asghari, B.; Dinparast, L.; Bahadori, S.; Moridi, F.M. Biological activities of Salvia santolinifolia Boiss. A multifunctional medicinal plant. Curr. Bioact. Compd., 2016, 12, 297-305.
[http://dx.doi.org/10.2174/1573407212666160426161112]
[26]
Lehbili, M.; Alabdul Magid, A.; Hubert, J.; Kabouche, A.; Voutquenne-Nazabadioko, L.; Renault, J.H.; Nuzillard, J.M.; Morjani, H.; Abedini, A.; Gangloff, S.C.; Kabouche, Z. Two new bis-iridoids isolated from Scabiosa stellata and their antibacterial, antioxidant, anti-tyrosinase and cytotoxic activities. Fitoterapia, 2018, 125, 41-48.
[http://dx.doi.org/10.1016/j.fitote.2017.12.018] [PMID: 29273413]
[27]
Lehbili, M.; Alabdul Magid, A.; Kabouche, A.; Voutquenne-Nazabadioko, L.; Morjani, H.; Harakat, D.; Kabouche, Z. Triterpenoid saponins from Scabiosa stellata collected in North-eastern Algeria. Phytochemistry, 2018, 150, 40-49.
[http://dx.doi.org/10.1016/j.phytochem.2018.03.005] [PMID: 29547709]
[28]
Rahmouni, N.; Pinto, D.C.; Santos, S.A.; Beghidja, N.; Silva, A.M. Lipophilic composition of Scabiosa stellata L.: An underexploited plant from Batna (Algeria). Chem. Pap., 2018, 72, 753-762.
[http://dx.doi.org/10.1007/s11696-017-0308-3]
[29]
Clarkson, C.; Maharaj, V.J.; Crouch, N.R.; Grace, O.M.; Pillay, P.; Matsabisa, M.G.; Bhagwandin, N.; Smith, P.J.; Folb, P.I. In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa. J. Ethnopharmacol., 2004, 92(2-3), 177-191.
[http://dx.doi.org/10.1016/j.jep.2004.02.011] [PMID: 15137999]
[30]
Zong, J.; Wang, R.; Bao, G.; Ling, T.; Zhang, L.; Zhang, X.; Hou, R. Novel triterpenoid saponins from residual seed cake of Camellia oleifera Abel. show anti-proliferative activity against tumor cells. Fitoterapia, 2015, 104, 7-13.
[http://dx.doi.org/10.1016/j.fitote.2015.05.001] [PMID: 25958771]
[31]
Cheng, L.; Xia, T.S.; Wang, Y.F.; Zhou, W.; Liang, X.Q.; Xue, J.Q.; Shi, L.; Wang, Y.; Ding, Q. The apoptotic effect of D Rhamnose β-hederin, a novel oleanane-type triterpenoid saponin on breast cancer cells. PLoS One, 2014, 9(6)e90848
[http://dx.doi.org/10.1371/journal.pone.0090848] [PMID: 24603880]
[32]
Gidaro, M.C.; Alcaro, F.; Carradori, S.; Costa, G.; Vullo, D.; Supuran, C.T.; Alcaro, S. Eriocitrin and apigenin as new carbonic anhydrase VA inhibitors, from a virtual screening of Calabrian natural products. Planta Med., 2015, 81(6), 533-540.
[http://dx.doi.org/10.1055/s-0034-1396139] [PMID: 25590364]
[33]
Vinutha, B.; Prashanth, D.; Salma, K.; Sreeja, S.L.; Pratiti, D.; Padmaja, R.; Radhika, S.; Amit, A.; Venkateshwarlu, K.; Deepak, M. Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. J. Ethnopharmacol., 2007, 109(2), 359-363.
[http://dx.doi.org/10.1016/j.jep.2006.06.014] [PMID: 16950584]
[34]
Szwajgier, D. Anticholinesterase activity of selected phenolic acids and flavonoids - interaction testing in model solutions. Ann. Agric. Environ. Med., 2015, 22(4), 690-694.
[http://dx.doi.org/10.5604/12321966.1185777] [PMID: 26706979]
[35]
Noudeh, G.D.; Sharififar, F.; Khatib, M.; Behravan, E.; Afzadi, M.A. Study of aqueous extract of three medicinal plants on cell membrane–permeabilizing and their surface properties. Afr. J. Biotechnol., 2010, 9, 110-116.
[36]
Sharma, O.P.; Kumar, N.; Singh, B.; Bhat, T.K. An improved method for thin layer chromatographic analysis of saponins. Food Chem., 2012, 132(1), 671-674.
[http://dx.doi.org/10.1016/j.foodchem.2011.10.069] [PMID: 26434348]
[37]
Voutquenne, L.; Lavaud, C.; Massiot, G.; Men-Olivier, L.L. Structure-activity relationships of haemolytic saponins. Pharm. Biol., 2002, 40, 253-262.
[http://dx.doi.org/10.1076/phbi.40.4.253.8470]
[38]
Zhang, Q.; Mao, Z.; Zhang, Q.; Qiu, J.; Jia, Z.; Qin, L. Acute and sub-chronic toxicological studies of the iridoid glycosides extract of Lamiophlomis rotata (Benth.) Kudo in rats. Regul. Toxicol. Pharmacol., 2018, 92, 315-323.
[http://dx.doi.org/10.1016/j.yrtph.2017.12.018] [PMID: 29287802]
[39]
Wang, M.; Guo, Q.; Xu, X.; Wang, X.; Ye, X.; Wu, S.; Hooper, D.C.; Wang, M. New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis. Antimicrob. Agents Chemother., 2009, 53(5), 1892-1897.
[http://dx.doi.org/10.1128/AAC.01400-08] [PMID: 19258263]
[40]
Benli, M.; Bingol, U.; Geven, F.; Guney, K.; Yigit, N. An investigation on the antimicrobial activity of some endemic plant species from Turkey. Afr. J. Biotechnol., 2008, 7, 1-5.
[41]
Najafi, S. Phytochemical screening and antibacterial activity of leaf extract of Ziziphus mauritiana Lam. Int. Res. J. Appl. Basic Sci., 2013, 4, 3274-3276.
[42]
Singh, B.A.; Sharma, R. Anti-inflammatory and antimicrobial effects of flavonoids from Heliotropium ellipticum exudate. Curr. Bioact. Compd., 2016, 12, 123-131.
[http://dx.doi.org/10.2174/157340721202160504223537]
[43]
Popova, V.; Gochev, V.; Girova, T.; Iliev, I.; Ivanova, T.; Stoyanova, A. Extraction products from tobacco-aroma and bioactive compounds and activities. Curr. Bioact. Compd., 2015, 11, 31-37.
[http://dx.doi.org/10.2174/157340721101150804150016]