Author(s): Jiejun Wu*, J.Eric McDuffie*, Jiao Song, Michael C. Harris, Holly Raymond, Yafei Chen, Leslie Nguyen, Steven Nguyen, Justin Kanerva, Heather M. McAllister, Xiaohu Deng, Neelakandha Mani, Changlu Liu and Siquan Sun
Page: [2781 - 2790] Pages: 10
* (Excluding Mailing and Handling)
We have developed a workflow to extract, separate, and semi-quantify bioactive oxysterols from mouse colon tissues and fecal matters using solid- and liquid-phase extractions, enzymatic and chemical modifications, and stable-isotope dilution LC/MS/MS. The method was applied to a dextran sodium sulphate (DSS)-induced mouse colitis model, which revealed that one particular dihydroxycholesterol (diOHC), 7α,25-diOHC, was significantly elevated in both colon tissue and fecal matters of mice with colitis compared to that in naïve mice. The extent of 7α,25-diOHC elevation was positively correlated with colitis severity.
Keywords: Oxysterol separation, Oxysterol quantification, Oxysterol analysis, Dihydroxycholesterols, Mouse colitis biomarker.
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