Abstract

Background: Lipid peroxidation induced by drug can be considered as drug induced toxicity. The concept of antioxidant co-therapy may be the way of future formulation design with an aim of reducing drug induced toxicity.

Objective: The current study was designed to evaluate the anti-peroxidative activity of quercetin and ascorbic acid on anti cancer drug (6-Mercaptopurine/Methotrexate) induced lipid peroxidation.

Method: Study was carried out by Reverse Phase HPLC method for the measurement of free malondialdehyde (a relevant biomarker for lipid peroxidation estimation) in goat liver. After the preparation of standard curve for malondialdehyde estimation, a tissue homogenate of goat liver was prepared and divided in to six different groups and were treated with the anti- cancer drugs and antioxidants. Then after, 2 hours of incubation, the supernatant was collected, filtered and procured to inject in RP-HPLC using mobile phase 30 mM potassium di hydrogen phosphate and Methanol (65:35, v/v %), at pH of 4.

Result: 6-Mercaptopurine/Methotrexate showed significant increase in malondialdehyde concentration (45.12% and 12.66 %) respectively as compared to control group. Wherever, concentration was significantly reduced in anticancer drugs in combination with antioxidants (41.74 %, -34.91 % and -26.96 %,-73.56 %). While, concentration of malondialdehyde (-75.01 %, 62.30 %) was reduced in antioxidant treated group in comparison to control and drug treated groups.

Conclusion: It was revealed that quercetin and ascorbic acid have beneficial effect to suppress or decrease the drug induced changes in lipid profile of liver samples.

Keywords: Free radical, antioxidants, anti cancer drug, lipid peroxidation, malondialdehyde, reverse phase HPLC.

Graphical Abstract