Abstract

The labeling of biomolecule using ^99mTc-tricarbonyl is an interesting tool for the diagnosis of diseases. This labeling startegy has several advantages as compared to other common radiolabeling techniques. This review is a complete overview of synthesis and chemistry of ^99mTc-tricarbonyl molecule for labeling peptides and proteins. Also, the effect of ligand type on the stability and in vivo biodistribution of ^99mTc-tricarbonyl labeled biomolecules are discussed. Chemistries of cyclopentadienyl and hexa histidine tag as two important bifunctional chelating agents (BFCA) are presented. The in vitro and in vivo behaviors of some ^99mTc-tricarbonyl labeled peptides and proteins are explained. Preclinical outcomes revealed that these labeled compounds are biologically, kinetically and thermodynamically stable. Findings showed that ^99mTc-tricarbonyl labeled biomolecules are promising tools for future clinical applications in image diagnosis.

Keywords: Tricarbonyl, ^99mTc, peptide, labeling.