Abstract

Labeo rohita (rohu) is one of the most important freshwater carps widely cultured in India, Bangladesh and other adjacent countries in the region. Proteomic analysis from liver tissue samples of riverine L. rohita was carried out with two-dimensional polyacrylamide gel electrophoresis (2-DE). On an average 450 spots were detected in a colloidal Coomassie stained 2-DE gel using PDQuest software. Forty two spots with high abundance and clear separation were selected from the gel and twelve of them were later analyzed with Electronspray Ionization (ESI) - Mass Spectrometry and identified by MASCOT search. The paper describes first attempt for protein profiling of liver tissue samples of L. rohita based on protocol standardized in our lab for optimized yield of total protein as well as 2-D electrophoresis.

Keywords: 2-D PAGE, Labeo rohita, Mass Spectrometry, Protein expression signatures.

Graphical Abstract