Abstract

Indirect resolution of chiral molecules, based upon pre-column derivatization and diastereomer formation using benzofurazan bearing chiral labeling reagents, by high-performance liquid chromatography are described in this minireview. The synthesis, characteristics and application of the fluorescent chiral derivatization reagents for various functional groups, i.e. amine (NBD-PyNCS, DBD-PyNCS, DBD-β-Pro, DBD-hydroxyproline), carboxyl (NBD-APy, DBD-APy, ABD-APy), carbonyl (NBD-ProCZ, DBD-ProCZ), hydroxyl (NBD-Pro-COCl, DBD-Pro-COCl) and thiol, etc., are including in the text.

Keywords: benzofurazan fluorophore, chiral resolution, biological important molecule, diastereomer formation, hplc analysis