Abstract

The investigation was carried out to determine the chemical composition of the fatty acids methyl esters (FAME) and leaf of Nyctanthes arbortristis was analyzed by gas liquid chromatography (GLC). GLC analyses of methyl ester lead to identify twenty two and fifteen fatty acids in the leaf of pet-ether and hexane extracts mixture (PH) and chloroform extract (CL), respectively. A Majority fatty acids, like palmitic acid (C16:0, 25.46%), arachidic acid (C20:0, 24.82%) and oleic acid (C18:1, 8.84%) were identified in leaf PH. Chloroform extract of FAME contains palmitic acid (C16:0, 23.9%), alfa-linolenic acid (C18:3, 15.11%) and oleic acid (C18:1, 14.98%) as major fatty acids in leaf. In this study, a new extraction method has been established.

Keywords: Seuli, leaf, fatty acid, combined method, Nyctanthes arbortristis, gas liquid chromatography