Abstract

Bioactivity of the cytoskeleton proteins such as actin, myosin and titin that have been extracted from the rabbit muscle was analyzed on plant misrospores as unicellular biosensors. The fibrillar forms of the proteins stimulated the germination of vegetative microspores from horsetail Equisetum arvense and generative (pollen) microspores from knight’s star Hippeastrum hybridum. In special experiments the proteins tested were able to reverse the blockade of ion channels by drugs d-tubocurarine and α-bungarotoxin, acting on cholinoreceptors, or verapamil, regulating Ca2+-channels.

Keywords: Actin, biosensors, Equisetum arvense, germination, Hippeastrum hybridum, ion channels’ blockers, myosin, pollen, titin.