Abstract

Glycoproteomic researches have been rapidly progressing in the last few years. Although intact glycopeptide analysis which obtains glycosite and corresponding glycan information simultaneously is still challenging, various studies, combining glycosylation enrichment methods and mass spectrometry techniques, have accumulated a considerable amount of protein glycosite data for either N- or O-glycosylation or O-GlcNAcylation. This review will majorly focus on recent research outputs in the field of glycosite exploration.

Keywords: Glycosite, enrichment, de-glycosylation, intact glycopeptides, quantification, collision-induced dissociation (CID), electron transfer dissociation (ETD), multistage tandem MS (MSn), Glycosite Analysis, Glycoproteomics, Mass Spectrometry, Glycosite, Enrichment, Collision-inducced dissociation (CID), Post-translational modifications, Covalent modifications, Numerous Cellular Processes, Glycoproteins, Post Proteomic er, N-linked glycans, Sugar structure elucidation, Micro-heterogeneity, Coexisting non-glycoproteins/peptides, Mammalian lectins, Galectins, selectins, Chemo-enzymatic labeling, De-Glycosylation Methods, O-GlcNAc modifcation, Beta-elemination, Michael addition, Dithiothreitol, Multiple lectin-mediated, Hydrazide chemistry, Non-redundant glycoproteins, Subproteome, O-GlcNAc Site, Mucin-type O-Glycosites, Electron Capture Dissociation (ECD), Infrared multiphoton dissociation (IRMPD), ETD/ECD Assisted Glycosite, Labeling based Quantification, Label-free Quantification, Immuno-enriched, BAP=Biotin pentylamine, ESI=Electrospray ionization, CDK=Climatic droplet keratopathy, CSC=Cell surface capturing, CRC=Colorectal cancer