Abstract

Identification of thiol modifications has gained significant importance. It is increasingly recognized that cysteines play an important role in protein function under both physiological and patho-physiological conditions. Here we reviewed different approaches that are used to identify oxidized proteins and discuss different fluorescent labeling techniques, differential two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization – time of flight identification, in short MALDI-TOF. We illuminate processes that depend on protein oxidation of cysteines and we look

Keywords: Aging, cysteine, oxidation, thiol, DIGE, MALDI-TOF, Western blot, dye labeling, disease