Abstract

Oligonucleotide analogs with L- and D-lysine residues incorporated in internucleotide linkages were synthesized and their affinity toward complementary DNA was studied. Stability of the duplexes formed by the modified oligonucleotides and their wild-type complements appeared to be close to that of the isosequential unmodified duplex, oligonucleotides carrying D-lysine residues forming generally more stable duplexes than L-lysine derivatives.

Keywords: Analogs, hybridization, oligonucleotides, peptide internucleotide linkages, synthesis, thermal stability, duplexes, triplexes, epimerization, dichloromethane