Abstract

Peptide arrays are useful tools to characterize antibodies, to determine sequence specificities of enzymes, or to find interaction partners to given peptide sequences. One widely-used format for such arrays is a cellulose sheet with hundreds of synthetic peptides bound to it. These SPOT arrays have been used successfully in a broad range of applications since their invention at the beginning of the nineties. The simplicity and robustness of this method along with the fully automated synthesis to generate custom arrays with high peptide densities made the SPOT method popular. A drawback of the SPOT method is the use of large reagent volumes and the limited throughput with only one copy of the library. CelluSpots represent a new method that retains the advantages of the SPOT method but allows the production of hundreds of identical copies on microscope slides for parallel screenings with low sample volumes.

Keywords: Antibody profiling, CelluSpots, kinase substrates, peptide arrays, peptide conjugates, protein interaction, SPOT