Abstract

Two arginine side-chain protecting groups, NG-4-methoxy-2,3,6-trimethylbenzensulfonyl group (Mtr) and NG- 2,2,5,7,8-pentamethylchroman-6-sulfonyl (Pmc), have been investigated at both the Arg1 and/or Arg9 position of the bioactive peptide, Bradykinin using Fluorenylmethyloxycarbonyl (Fmoc) Solid Phase Peptide Synthesis. A more efficient synthesis of the peptide has been found when a combination of Arg(Mtr) is present at position 1 and Arg(Pmc) is present at position 9 giving a cleaved pure yield of 52%.

Keywords: Bradykinin, drug delivery, fmoc solid phase peptide synthesis, RP-HPLC, TBTU, DMF, ninhydrin test, IEA, NMR spectroscopy, Fmoc-L-amino acids, TOF, Acetonitrile, EDT, Rink resin, Phenomenexguard columnBradykinin, drug delivery, fmoc solid phase peptide synthesis, RP-HPLC, TBTU, DMF, ninhydrin test, IEA, NMR spectroscopy, Fmoc-L-amino acids, TOF, Acetonitrile, EDT, Rink resin, Phenomenexguard column