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Protein & Peptide Letters

Author(s): Alessia Ruggiero, Flavia Squeglia, Viviana Izzo, Alba Silipo, Luigi Vitagliano, Antonio Molinaro and Rita Berisio

DOI: 10.2174/092986610792231401

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Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Peptidoglycan Binding Region of the Ser/Thr Kinase PrkC from Staphylococcus aureus

Page: [1296 - 1299] Pages: 4

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Abstract

PrkC is an important Ser/Thr membrane kinase of Staphylococcus aureus able to bind peptidoglycans through extra-cellular domains, denominated as PASTA. Upon peptidoglycan binding, PrkC is activated and stimulates bacterial growth and revival from latency. The entire extra-cellular region of PrkC (residues 378-664), containing three predicted PASTA domains and an extra-domain of unknown function, has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and refinement is in progress.

Keywords: Crystal, cell wall, X-ray, latency